Publication

Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy

Stavenga, D. G., Leertouwer, H. L. & Wilts, B. D., Sep-2013, In : Light-Science & applications. 2, 9, 6 p., E100.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Stavenga, D. G., Leertouwer, H. L., & Wilts, B. D. (2013). Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy. Light-Science & applications, 2(9), [E100]. https://doi.org/10.1038/lsa.2013.56

Author

Stavenga, Doekele G. ; Leertouwer, Hein L. ; Wilts, Bodo D. / Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy. In: Light-Science & applications. 2013 ; Vol. 2, No. 9.

Harvard

Stavenga, DG, Leertouwer, HL & Wilts, BD 2013, 'Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy', Light-Science & applications, vol. 2, no. 9, E100. https://doi.org/10.1038/lsa.2013.56

Standard

Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy. / Stavenga, Doekele G.; Leertouwer, Hein L.; Wilts, Bodo D.

In: Light-Science & applications, Vol. 2, No. 9, E100, 09.2013.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Stavenga DG, Leertouwer HL, Wilts BD. Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy. Light-Science & applications. 2013 Sep;2(9). E100. https://doi.org/10.1038/lsa.2013.56


BibTeX

@article{915f6e46c30e411e89f90f07cc95c1be,
title = "Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy",
abstract = "Jamin-Lebedeff polarizing interference microscopy is a classical method for determining the refractive index and thickness of transparent tissues. Here, we extend the application of this method to pigmented, absorbing biological tissues, based on a theoretical derivation using Jones calculus. This novel method is applied to the wings of the American Rubyspot damselfly, Hetaerina americana. The membranes in the red-colored parts of the damselfly's wings, with a thickness of similar to 2.5 mu m, contain a pigment with maximal absorption at similar to 490 nm and a peak absorbance coefficient of similar to 0.7 mu m(-1). The high pigment density causes a considerable and anomalous dispersion of the refractive index. This result can be quantitatively understood from the pigment absorbance spectrum by applying the Kramers-Kronig dispersion relations. Measurements of the spectral dependence of the refractive index and the absorption are valuable for gaining quantitative insight into how the material properties of animal tissues influence coloration.",
keywords = "absorption, chitin, insect wings, Jones calculus, Kramers-Kronig relations, STRUCTURAL COLORS, COLORATION, ABSORBENCY, LIGHT",
author = "Stavenga, {Doekele G.} and Leertouwer, {Hein L.} and Wilts, {Bodo D.}",
year = "2013",
month = "9",
doi = "10.1038/lsa.2013.56",
language = "English",
volume = "2",
journal = "Light-Science & applications",
issn = "2047-7538",
publisher = "CHINESE ACAD SCIENCES, CHANGCHUN INST OPTICS FINE MECHANICS AND PHYSICS",
number = "9",

}

RIS

TY - JOUR

T1 - Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin-Lebedeff interference microscopy

AU - Stavenga, Doekele G.

AU - Leertouwer, Hein L.

AU - Wilts, Bodo D.

PY - 2013/9

Y1 - 2013/9

N2 - Jamin-Lebedeff polarizing interference microscopy is a classical method for determining the refractive index and thickness of transparent tissues. Here, we extend the application of this method to pigmented, absorbing biological tissues, based on a theoretical derivation using Jones calculus. This novel method is applied to the wings of the American Rubyspot damselfly, Hetaerina americana. The membranes in the red-colored parts of the damselfly's wings, with a thickness of similar to 2.5 mu m, contain a pigment with maximal absorption at similar to 490 nm and a peak absorbance coefficient of similar to 0.7 mu m(-1). The high pigment density causes a considerable and anomalous dispersion of the refractive index. This result can be quantitatively understood from the pigment absorbance spectrum by applying the Kramers-Kronig dispersion relations. Measurements of the spectral dependence of the refractive index and the absorption are valuable for gaining quantitative insight into how the material properties of animal tissues influence coloration.

AB - Jamin-Lebedeff polarizing interference microscopy is a classical method for determining the refractive index and thickness of transparent tissues. Here, we extend the application of this method to pigmented, absorbing biological tissues, based on a theoretical derivation using Jones calculus. This novel method is applied to the wings of the American Rubyspot damselfly, Hetaerina americana. The membranes in the red-colored parts of the damselfly's wings, with a thickness of similar to 2.5 mu m, contain a pigment with maximal absorption at similar to 490 nm and a peak absorbance coefficient of similar to 0.7 mu m(-1). The high pigment density causes a considerable and anomalous dispersion of the refractive index. This result can be quantitatively understood from the pigment absorbance spectrum by applying the Kramers-Kronig dispersion relations. Measurements of the spectral dependence of the refractive index and the absorption are valuable for gaining quantitative insight into how the material properties of animal tissues influence coloration.

KW - absorption

KW - chitin

KW - insect wings

KW - Jones calculus

KW - Kramers-Kronig relations

KW - STRUCTURAL COLORS

KW - COLORATION

KW - ABSORBENCY

KW - LIGHT

U2 - 10.1038/lsa.2013.56

DO - 10.1038/lsa.2013.56

M3 - Article

VL - 2

JO - Light-Science & applications

JF - Light-Science & applications

SN - 2047-7538

IS - 9

M1 - E100

ER -

ID: 5979074