Publication

Quantification of Aneuploidy in Mammalian Systems

van den Bos, H., Bakker, B., Taudt, A., Guryev, V., Colomé-Tatché, M., Lansdorp, P. M., Foijer, F. & Spierings, D. C. J., 25-Nov-2018, In : Methods in Molecular Biology. 1896, p. 159-190 32 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

van den Bos, H., Bakker, B., Taudt, A., Guryev, V., Colomé-Tatché, M., Lansdorp, P. M., ... Spierings, D. C. J. (2018). Quantification of Aneuploidy in Mammalian Systems. Methods in Molecular Biology, 1896, 159-190. https://doi.org/10.1007/978-1-4939-8931-7_15

Author

van den Bos, Hilda ; Bakker, Bjorn ; Taudt, Aaron ; Guryev, Victor ; Colomé-Tatché, Maria ; Lansdorp, Peter M ; Foijer, Floris ; Spierings, Diana C J. / Quantification of Aneuploidy in Mammalian Systems. In: Methods in Molecular Biology. 2018 ; Vol. 1896. pp. 159-190.

Harvard

van den Bos, H, Bakker, B, Taudt, A, Guryev, V, Colomé-Tatché, M, Lansdorp, PM, Foijer, F & Spierings, DCJ 2018, 'Quantification of Aneuploidy in Mammalian Systems', Methods in Molecular Biology, vol. 1896, pp. 159-190. https://doi.org/10.1007/978-1-4939-8931-7_15

Standard

Quantification of Aneuploidy in Mammalian Systems. / van den Bos, Hilda; Bakker, Bjorn; Taudt, Aaron; Guryev, Victor; Colomé-Tatché, Maria; Lansdorp, Peter M; Foijer, Floris; Spierings, Diana C J.

In: Methods in Molecular Biology, Vol. 1896, 25.11.2018, p. 159-190.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

van den Bos H, Bakker B, Taudt A, Guryev V, Colomé-Tatché M, Lansdorp PM et al. Quantification of Aneuploidy in Mammalian Systems. Methods in Molecular Biology. 2018 Nov 25;1896:159-190. https://doi.org/10.1007/978-1-4939-8931-7_15


BibTeX

@article{5f2b29b51f7b4c3a8635fa6beec64b57,
title = "Quantification of Aneuploidy in Mammalian Systems",
abstract = "High-throughput next generation sequencing karyotyping has emerged as a powerful tool for the detection of genomic heterogeneity in normal tissues and cancers. Here we describe a single-cell whole genome sequencing (scWGS) platform to assess whole-chromosome aneuploidy, structural aneuploidies involving only chromosome fragments and more local small copy number alterations in individual cells. We provide a detailed protocol for the isolation, library preparation, low coverage sequencing and data analysis of single cells. Since our approach does not involve a whole-genome preamplification step, our method allows for acquisition of reliable high-resolution single-cell copy number profiles. Moreover, the protocol allows multiplexing of 384 single-cell libraries in one sequencing run, thereby significantly reducing sequencing costs and can be completed in 3-4 days starting from single cell isolation to analysis of sequencing data.",
author = "{van den Bos}, Hilda and Bjorn Bakker and Aaron Taudt and Victor Guryev and Maria Colom{\'e}-Tatch{\'e} and Lansdorp, {Peter M} and Floris Foijer and Spierings, {Diana C J}",
year = "2018",
month = "11",
day = "25",
doi = "10.1007/978-1-4939-8931-7_15",
language = "English",
volume = "1896",
pages = "159--190",
journal = "Methods in Molecular Biology",
issn = "1064-3745",

}

RIS

TY - JOUR

T1 - Quantification of Aneuploidy in Mammalian Systems

AU - van den Bos, Hilda

AU - Bakker, Bjorn

AU - Taudt, Aaron

AU - Guryev, Victor

AU - Colomé-Tatché, Maria

AU - Lansdorp, Peter M

AU - Foijer, Floris

AU - Spierings, Diana C J

PY - 2018/11/25

Y1 - 2018/11/25

N2 - High-throughput next generation sequencing karyotyping has emerged as a powerful tool for the detection of genomic heterogeneity in normal tissues and cancers. Here we describe a single-cell whole genome sequencing (scWGS) platform to assess whole-chromosome aneuploidy, structural aneuploidies involving only chromosome fragments and more local small copy number alterations in individual cells. We provide a detailed protocol for the isolation, library preparation, low coverage sequencing and data analysis of single cells. Since our approach does not involve a whole-genome preamplification step, our method allows for acquisition of reliable high-resolution single-cell copy number profiles. Moreover, the protocol allows multiplexing of 384 single-cell libraries in one sequencing run, thereby significantly reducing sequencing costs and can be completed in 3-4 days starting from single cell isolation to analysis of sequencing data.

AB - High-throughput next generation sequencing karyotyping has emerged as a powerful tool for the detection of genomic heterogeneity in normal tissues and cancers. Here we describe a single-cell whole genome sequencing (scWGS) platform to assess whole-chromosome aneuploidy, structural aneuploidies involving only chromosome fragments and more local small copy number alterations in individual cells. We provide a detailed protocol for the isolation, library preparation, low coverage sequencing and data analysis of single cells. Since our approach does not involve a whole-genome preamplification step, our method allows for acquisition of reliable high-resolution single-cell copy number profiles. Moreover, the protocol allows multiplexing of 384 single-cell libraries in one sequencing run, thereby significantly reducing sequencing costs and can be completed in 3-4 days starting from single cell isolation to analysis of sequencing data.

U2 - 10.1007/978-1-4939-8931-7_15

DO - 10.1007/978-1-4939-8931-7_15

M3 - Article

VL - 1896

SP - 159

EP - 190

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 71379541