Quantification of Aneuploidy in Mammalian Systems

van den Bos, H., Bakker, B., Taudt, A., Guryev, V., Colomé-Tatché, M., Lansdorp, P. M., Foijer, F. & Spierings, D. C. J., 25-Nov-2018, Springer Protocols. 32 p. (Methods in Molecular Biology)

Research output: Book/ReportProtocolProfessional

High-throughput next generation sequencing karyotyping has emerged as a powerful tool for the detection of genomic heterogeneity in normal tissues and cancers. Here we describe a single-cell whole genome sequencing (scWGS) platform to assess whole-chromosome aneuploidy, structural aneuploidies involving only chromosome fragments and more local small copy number alterations in individual cells. We provide a detailed protocol for the isolation, library preparation, low coverage sequencing and data analysis of single cells. Since our approach does not involve a whole-genome preamplification step, our method allows for acquisition of reliable high-resolution single-cell copy number profiles. Moreover, the protocol allows multiplexing of 384 single-cell libraries in one sequencing run, thereby significantly reducing sequencing costs and can be completed in 3-4 days starting from single cell isolation to analysis of sequencing data.

Original languageEnglish
PublisherSpringer Protocols
Number of pages32
ISBN (Electronic)978-1-4939-8931-7
ISBN (Print)978-1-4939-8930-0
Publication statusE-pub ahead of print - 25-Nov-2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

ID: 121494216