Publication

Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri

Kopacz, M. M., Lorenzoni, A. S. G., Polaquini, C. R., Regasini, L. O. & Scheffers, D-J., May-2019, In : MicrobiologyOpen. 8, 5, 11 p., e00706.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Kopacz, M. M., Lorenzoni, A. S. G., Polaquini, C. R., Regasini, L. O., & Scheffers, D-J. (2019). Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri. MicrobiologyOpen, 8(5), [e00706]. https://doi.org/10.1002/mbo3.706

Author

Kopacz, Malgorzata M ; Lorenzoni, André S G ; Polaquini, Carlos R ; Regasini, Luis O ; Scheffers, Dirk-Jan. / Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri. In: MicrobiologyOpen. 2019 ; Vol. 8, No. 5.

Harvard

Kopacz, MM, Lorenzoni, ASG, Polaquini, CR, Regasini, LO & Scheffers, D-J 2019, 'Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri', MicrobiologyOpen, vol. 8, no. 5, e00706. https://doi.org/10.1002/mbo3.706

Standard

Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri. / Kopacz, Malgorzata M; Lorenzoni, André S G; Polaquini, Carlos R; Regasini, Luis O; Scheffers, Dirk-Jan.

In: MicrobiologyOpen, Vol. 8, No. 5, e00706, 05.2019.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Kopacz MM, Lorenzoni ASG, Polaquini CR, Regasini LO, Scheffers D-J. Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri. MicrobiologyOpen. 2019 May;8(5). e00706. https://doi.org/10.1002/mbo3.706


BibTeX

@article{d372af894081464ab245678dfa28ef7f,
title = "Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri",
abstract = "Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds.",
keywords = "antibacterials, cell division, FtsZ, gallates, gallic acid, GTPase, membrane permeabilization",
author = "Kopacz, {Malgorzata M} and Lorenzoni, {Andr{\'e} S G} and Polaquini, {Carlos R} and Regasini, {Luis O} and Dirk-Jan Scheffers",
note = "{\textcopyright} 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",
year = "2019",
month = may,
doi = "10.1002/mbo3.706",
language = "English",
volume = "8",
journal = "MicrobiologyOpen",
issn = "2045-8827",
publisher = "Wiley",
number = "5",

}

RIS

TY - JOUR

T1 - Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri

AU - Kopacz, Malgorzata M

AU - Lorenzoni, André S G

AU - Polaquini, Carlos R

AU - Regasini, Luis O

AU - Scheffers, Dirk-Jan

N1 - © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

PY - 2019/5

Y1 - 2019/5

N2 - Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds.

AB - Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds.

KW - antibacterials

KW - cell division

KW - FtsZ

KW - gallates

KW - gallic acid

KW - GTPase

KW - membrane permeabilization

U2 - 10.1002/mbo3.706

DO - 10.1002/mbo3.706

M3 - Article

C2 - 30085414

VL - 8

JO - MicrobiologyOpen

JF - MicrobiologyOpen

SN - 2045-8827

IS - 5

M1 - e00706

ER -

ID: 63747466