Publication

Purification and characterization of FtsZ from the citrus canker pathogen Xanthomonas citri subsp. citri

Kopacz, M. M., Lorenzoni, A. S. G., Polaquini, C. R., Regasini, L. O. & Scheffers, D-J., May-2019, In : MicrobiologyOpen. 8, 5, 11 p., e00706.

Research output: Contribution to journalArticleAcademicpeer-review

  • Malgorzata M Kopacz
  • André S G Lorenzoni
  • Carlos R Polaquini
  • Luis O Regasini
  • Dirk-Jan Scheffers

Xanthomonas citri subsp. citri (Xac) is the causative agent of citrus canker, a plant disease that significantly impacts citriculture. In earlier work, we showed that alkylated derivatives of gallic acid have antibacterial action against Xac and target both the cell division protein FtsZ and membrane integrity in Bacillus subtilis. Here, we have purified native XacFtsZ and characterized its GTP hydrolysis and polymerization properties. In a surprising manner, inhibition of XacFtsZ activity by alkyl gallates is not as strong as observed earlier with B. subtilis FtsZ. As the alkyl gallates efficiently permeabilize Xac membranes, we propose that this is the primary mode of antibacterial action of these compounds.

Original languageEnglish
Article numbere00706
Number of pages11
JournalMicrobiologyOpen
Volume8
Issue number5
Early online date7-Aug-2018
Publication statusPublished - May-2019

    Keywords

  • antibacterials, cell division, FtsZ, gallates, gallic acid, GTPase, membrane permeabilization

ID: 63747466