Pulmonary delivery of an inulin-stabilized influenza subunit vaccine prepared by spray-freeze drying induces systemic, mucosal humoral as well as cell-mediated immune responses in BALB/c mice

Amorij, J-P., Saluja, V., Petersen, A. H., Hinrichs, W. L. J., Huckriede, A. & Frijlink, H. W., 17-Dec-2007, In : Vaccine. 25, 52, p. 8707-8717 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

In this study pulmonary vaccination with a new influenza subunit vaccine powder was evaluated. Vaccine powder was produced by spray-freeze drying (SFD) using the oligosaccharide inulin as stabilizer. Immune responses after pulmonary vaccination of BALB/c mice with vaccine powder were determined and compared to those induced by intramuscular and pulmonary vaccination with a conventional liquid subunit vaccine. All vaccinations were performed without adjuvant. Pulmonary vaccination with liquid subunit vaccine resulted in systemic Immoral (IgG) immune responses similar to intramuscular immunization. In contrast, the vaccine powder delivered by the pulmonary route, induced not only systemic Immoral (IgG) responses, but also cell-mediated (II-4, IFN-gamma) and mucosal immune responses (IgA, IgG). This study demonstrates that the combination of pulmonary antigen delivery and antigen powder production by SFD improves the immunogenic potential of (influenza subunit) antigen. In conclusion, vaccination with a non-adjuvanted SFD subunit vaccine powder by inhalation might be feasible and could be an alternative to conventional parenteral vaccine administration. (c) 2007 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)8707-8717
Number of pages11
Issue number52
Publication statusPublished - 17-Dec-2007


  • Dry powder formulation, Influenza vaccine, Respiratory tract, gamma interferon, immunoglobulin A, immunoglobulin G, influenza vaccine, interleukin 4, inulin, oligosaccharide, stabilizing agent, subunit vaccine, aerosol, animal cell, animal experiment, animal model, antibody response, article, Bagg albino mouse, cellular immunity, controlled study, cytokine production, drug delivery system, female, freeze drying, humoral immunity, immune response, immunogenicity, influenza, influenza vaccination, mouse, mucosal immunity, nonhuman, powder, priority journal, vaccine production

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