Publication

Proteomic alterations in early stage cervical cancer

Güzel, C., Govorukhina, N., Wisman, G. B. A., Stingl, C., Dekker, L., Hollema, H., Guryev, V., Horvatovich, P., van der Zee, A., Bischoff, R. & Luider, T., 6-Apr-2018, In : Oncotarget. 9, p. 18128-18147 20 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Güzel, C., Govorukhina, N., Wisman, G. B. A., Stingl, C., Dekker, L., Hollema, H., ... Luider, T. (2018). Proteomic alterations in early stage cervical cancer. Oncotarget, 9, 18128-18147. https://doi.org/10.18632/oncotarget.24773

Author

Güzel, Coşkun ; Govorukhina, Natalia ; Wisman, G.B.A. ; Stingl, Christoph ; Dekker, Lennard ; Hollema, Harry ; Guryev, Victor ; Horvatovich, Peter ; van der Zee, Ate ; Bischoff, Rainer ; Luider, Theo. / Proteomic alterations in early stage cervical cancer. In: Oncotarget. 2018 ; Vol. 9. pp. 18128-18147.

Harvard

Güzel, C, Govorukhina, N, Wisman, GBA, Stingl, C, Dekker, L, Hollema, H, Guryev, V, Horvatovich, P, van der Zee, A, Bischoff, R & Luider, T 2018, 'Proteomic alterations in early stage cervical cancer', Oncotarget, vol. 9, pp. 18128-18147. https://doi.org/10.18632/oncotarget.24773

Standard

Proteomic alterations in early stage cervical cancer. / Güzel, Coşkun; Govorukhina, Natalia; Wisman, G.B.A.; Stingl, Christoph; Dekker, Lennard; Hollema, Harry; Guryev, Victor; Horvatovich, Peter; van der Zee, Ate; Bischoff, Rainer; Luider, Theo.

In: Oncotarget, Vol. 9, 06.04.2018, p. 18128-18147.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Güzel C, Govorukhina N, Wisman GBA, Stingl C, Dekker L, Hollema H et al. Proteomic alterations in early stage cervical cancer. Oncotarget. 2018 Apr 6;9:18128-18147. https://doi.org/10.18632/oncotarget.24773


BibTeX

@article{5585d822aed240d08ccac1309a4ece75,
title = "Proteomic alterations in early stage cervical cancer",
abstract = "Laser capture microdissection (LCM) allows the capture of cell types or well-defined structures in tissue. We compared in a semi-quantitative way the proteomes from an equivalent of 8,000 tumor cells from patients with squamous cell cervical cancer (SCC, n = 22) with healthy epithelial and stromal cells obtained from normal cervical tissue (n = 13). Proteins were enzymatically digested into peptides which were measured by high-resolution mass spectrometry and analyzed by “all-or-nothing” analysis, Bonferroni, and Benjamini-Hochberg correction for multiple testing. By comparing LCM cell type preparations, 31 proteins were exclusively found in early stage cervical cancer (n = 11) when compared with healthy epithelium and stroma, based on criteria that address specificity in a restrictive “all-or-nothing” way. By Bonferroni correction for multiple testing, 30 proteins were significantly up-regulated between early stage cervical cancer and healthy control, including six members of the MCM protein family. MCM proteins are involved in DNA repair and expected to be participating in the early stage of cancer. After a less stringent Benjamini-Hochberg correction for multiple testing, we found that the abundances of 319 proteins were significantly different between early stage cervical cancer and healthy controls. Four proteins were confirmed in digests of whole tissue lysates by Parallel Reaction Monitoring (PRM). Ingenuity Pathway Analysis using correction for multiple testing by permutation resulted in two networks that were differentially regulated in early stage cervical cancer compared with healthy tissue. From these networks, we learned that specific tumor mechanisms become effective during the early stage of cervical cancer.",
author = "Coşkun G{\"u}zel and Natalia Govorukhina and G.B.A. Wisman and Christoph Stingl and Lennard Dekker and Harry Hollema and Victor Guryev and Peter Horvatovich and {van der Zee}, Ate and Rainer Bischoff and Theo Luider",
year = "2018",
month = "4",
day = "6",
doi = "10.18632/oncotarget.24773",
language = "English",
volume = "9",
pages = "18128--18147",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",

}

RIS

TY - JOUR

T1 - Proteomic alterations in early stage cervical cancer

AU - Güzel, Coşkun

AU - Govorukhina, Natalia

AU - Wisman, G.B.A.

AU - Stingl, Christoph

AU - Dekker, Lennard

AU - Hollema, Harry

AU - Guryev, Victor

AU - Horvatovich, Peter

AU - van der Zee, Ate

AU - Bischoff, Rainer

AU - Luider, Theo

PY - 2018/4/6

Y1 - 2018/4/6

N2 - Laser capture microdissection (LCM) allows the capture of cell types or well-defined structures in tissue. We compared in a semi-quantitative way the proteomes from an equivalent of 8,000 tumor cells from patients with squamous cell cervical cancer (SCC, n = 22) with healthy epithelial and stromal cells obtained from normal cervical tissue (n = 13). Proteins were enzymatically digested into peptides which were measured by high-resolution mass spectrometry and analyzed by “all-or-nothing” analysis, Bonferroni, and Benjamini-Hochberg correction for multiple testing. By comparing LCM cell type preparations, 31 proteins were exclusively found in early stage cervical cancer (n = 11) when compared with healthy epithelium and stroma, based on criteria that address specificity in a restrictive “all-or-nothing” way. By Bonferroni correction for multiple testing, 30 proteins were significantly up-regulated between early stage cervical cancer and healthy control, including six members of the MCM protein family. MCM proteins are involved in DNA repair and expected to be participating in the early stage of cancer. After a less stringent Benjamini-Hochberg correction for multiple testing, we found that the abundances of 319 proteins were significantly different between early stage cervical cancer and healthy controls. Four proteins were confirmed in digests of whole tissue lysates by Parallel Reaction Monitoring (PRM). Ingenuity Pathway Analysis using correction for multiple testing by permutation resulted in two networks that were differentially regulated in early stage cervical cancer compared with healthy tissue. From these networks, we learned that specific tumor mechanisms become effective during the early stage of cervical cancer.

AB - Laser capture microdissection (LCM) allows the capture of cell types or well-defined structures in tissue. We compared in a semi-quantitative way the proteomes from an equivalent of 8,000 tumor cells from patients with squamous cell cervical cancer (SCC, n = 22) with healthy epithelial and stromal cells obtained from normal cervical tissue (n = 13). Proteins were enzymatically digested into peptides which were measured by high-resolution mass spectrometry and analyzed by “all-or-nothing” analysis, Bonferroni, and Benjamini-Hochberg correction for multiple testing. By comparing LCM cell type preparations, 31 proteins were exclusively found in early stage cervical cancer (n = 11) when compared with healthy epithelium and stroma, based on criteria that address specificity in a restrictive “all-or-nothing” way. By Bonferroni correction for multiple testing, 30 proteins were significantly up-regulated between early stage cervical cancer and healthy control, including six members of the MCM protein family. MCM proteins are involved in DNA repair and expected to be participating in the early stage of cancer. After a less stringent Benjamini-Hochberg correction for multiple testing, we found that the abundances of 319 proteins were significantly different between early stage cervical cancer and healthy controls. Four proteins were confirmed in digests of whole tissue lysates by Parallel Reaction Monitoring (PRM). Ingenuity Pathway Analysis using correction for multiple testing by permutation resulted in two networks that were differentially regulated in early stage cervical cancer compared with healthy tissue. From these networks, we learned that specific tumor mechanisms become effective during the early stage of cervical cancer.

U2 - 10.18632/oncotarget.24773

DO - 10.18632/oncotarget.24773

M3 - Article

VL - 9

SP - 18128

EP - 18147

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

ER -

ID: 56880266