Publication

Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells

Van Os, R., Van Schie, MLJ., Willemze, R. & Fibbe, WE., Jul-2004, In : Journal of hematotherapy & stem cell research. 11, 3, p. 513-521 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Van Os, R., Van Schie, MLJ., Willemze, R., & Fibbe, WE. (2004). Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells. Journal of hematotherapy & stem cell research, 11(3), 513-521. https://doi.org/10.1089/15258160260090979

Author

Van Os, R ; Van Schie, MLJ ; Willemze, R ; Fibbe, WE. / Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells. In: Journal of hematotherapy & stem cell research. 2004 ; Vol. 11, No. 3. pp. 513-521.

Harvard

Van Os, R, Van Schie, MLJ, Willemze, R & Fibbe, WE 2004, 'Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells', Journal of hematotherapy & stem cell research, vol. 11, no. 3, pp. 513-521. https://doi.org/10.1089/15258160260090979

Standard

Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells. / Van Os, R; Van Schie, MLJ; Willemze, R; Fibbe, WE.

In: Journal of hematotherapy & stem cell research, Vol. 11, No. 3, 07.2004, p. 513-521.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Van Os R, Van Schie MLJ, Willemze R, Fibbe WE. Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells. Journal of hematotherapy & stem cell research. 2004 Jul;11(3):513-521. https://doi.org/10.1089/15258160260090979


BibTeX

@article{6a7738508ae64d0d876d75098f4e5b0c,
title = "Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells",
abstract = "Previous studies from our laboratory indicate that functional, mature neutrophils are essential for interleukin-8 (IL-8)-induced stem cell mobilization. To study a possible role of neutrophils in granulocyte colony-stimulating factor (G-CSF) induced hematopoietic mobilization, we assessed the number of circulating CD34(+) cells in healthy allogeneic stem cell donors on days 3, 4, and 5 of mobilization for comparison with the number of peripheral blood neutrophils and the plasma levels of IL-8, Flt3 ligand (FL), matrix metalloproteinase-9 (MMP-9), and human neutrophil elastase (HNE). Thirty-seven of 45 donors required 1 day of apheresis to obtain 5 x 10(6) CD34(+)/kg recipient body weight (high responders), the remaining 8 donors required I extra day of apheresis on day 6 (low responders). On day 5, CD34(+) numbers in the blood were significantly higher in high responders (116 x 10(3) +/- 10.4/ml) than in low responders (54.1 x 10(3) +/- 10.3, p <0.001). In all donors, MMP-9 and HNE levels were increased compared to nonmobilized individuals, but in high responders, plasma MMP-9 levels on days 3-5 of mobilization were substantially higher than in low responders (p &LE; 0.02 for MMP-9 and p = 0.89, p = 0.05 and p = 0.52 for HNE on days 3, 4, and 5, respectively). These results are in accordance with the hypothesis that neutrophils play a role in G-CSF-induced mobilization through the release of proteases such as MMP-9 and elastase. No change in plasma levels of IL-8 or Flt3 ligand was observed, suggesting that these cytokines do not play a role in stem cell mobilization. However, because stem cell numbers could not be predicted by proteolytic enzyme levels and/or neutrophil numbers, other undefined factors may be more important.",
keywords = "PERIPHERAL-BLOOD, PROGENITOR CELLS, CD34(+) CELLS, ADHESION MOLECULES, HUMAN NEUTROPHILS, PLASMA-LEVELS, INTERLEUKIN-8, ANTIBODIES, MARROW, METALLOPROTEINASES",
author = "{Van Os}, R and {Van Schie}, MLJ and R Willemze and WE Fibbe",
year = "2004",
month = "7",
doi = "10.1089/15258160260090979",
language = "English",
volume = "11",
pages = "513--521",
journal = "Journal of hematotherapy & stem cell research",
issn = "1525-8165",
number = "3",

}

RIS

TY - JOUR

T1 - Proteolytic enzyme levels are increased during granulocyte colony-stimulating factor-induced hematopoietic stem cell mobilization in human donors but do not predict the number of mobilized stem cells

AU - Van Os, R

AU - Van Schie, MLJ

AU - Willemze, R

AU - Fibbe, WE

PY - 2004/7

Y1 - 2004/7

N2 - Previous studies from our laboratory indicate that functional, mature neutrophils are essential for interleukin-8 (IL-8)-induced stem cell mobilization. To study a possible role of neutrophils in granulocyte colony-stimulating factor (G-CSF) induced hematopoietic mobilization, we assessed the number of circulating CD34(+) cells in healthy allogeneic stem cell donors on days 3, 4, and 5 of mobilization for comparison with the number of peripheral blood neutrophils and the plasma levels of IL-8, Flt3 ligand (FL), matrix metalloproteinase-9 (MMP-9), and human neutrophil elastase (HNE). Thirty-seven of 45 donors required 1 day of apheresis to obtain 5 x 10(6) CD34(+)/kg recipient body weight (high responders), the remaining 8 donors required I extra day of apheresis on day 6 (low responders). On day 5, CD34(+) numbers in the blood were significantly higher in high responders (116 x 10(3) +/- 10.4/ml) than in low responders (54.1 x 10(3) +/- 10.3, p <0.001). In all donors, MMP-9 and HNE levels were increased compared to nonmobilized individuals, but in high responders, plasma MMP-9 levels on days 3-5 of mobilization were substantially higher than in low responders (p &LE; 0.02 for MMP-9 and p = 0.89, p = 0.05 and p = 0.52 for HNE on days 3, 4, and 5, respectively). These results are in accordance with the hypothesis that neutrophils play a role in G-CSF-induced mobilization through the release of proteases such as MMP-9 and elastase. No change in plasma levels of IL-8 or Flt3 ligand was observed, suggesting that these cytokines do not play a role in stem cell mobilization. However, because stem cell numbers could not be predicted by proteolytic enzyme levels and/or neutrophil numbers, other undefined factors may be more important.

AB - Previous studies from our laboratory indicate that functional, mature neutrophils are essential for interleukin-8 (IL-8)-induced stem cell mobilization. To study a possible role of neutrophils in granulocyte colony-stimulating factor (G-CSF) induced hematopoietic mobilization, we assessed the number of circulating CD34(+) cells in healthy allogeneic stem cell donors on days 3, 4, and 5 of mobilization for comparison with the number of peripheral blood neutrophils and the plasma levels of IL-8, Flt3 ligand (FL), matrix metalloproteinase-9 (MMP-9), and human neutrophil elastase (HNE). Thirty-seven of 45 donors required 1 day of apheresis to obtain 5 x 10(6) CD34(+)/kg recipient body weight (high responders), the remaining 8 donors required I extra day of apheresis on day 6 (low responders). On day 5, CD34(+) numbers in the blood were significantly higher in high responders (116 x 10(3) +/- 10.4/ml) than in low responders (54.1 x 10(3) +/- 10.3, p <0.001). In all donors, MMP-9 and HNE levels were increased compared to nonmobilized individuals, but in high responders, plasma MMP-9 levels on days 3-5 of mobilization were substantially higher than in low responders (p &LE; 0.02 for MMP-9 and p = 0.89, p = 0.05 and p = 0.52 for HNE on days 3, 4, and 5, respectively). These results are in accordance with the hypothesis that neutrophils play a role in G-CSF-induced mobilization through the release of proteases such as MMP-9 and elastase. No change in plasma levels of IL-8 or Flt3 ligand was observed, suggesting that these cytokines do not play a role in stem cell mobilization. However, because stem cell numbers could not be predicted by proteolytic enzyme levels and/or neutrophil numbers, other undefined factors may be more important.

KW - PERIPHERAL-BLOOD

KW - PROGENITOR CELLS

KW - CD34(+) CELLS

KW - ADHESION MOLECULES

KW - HUMAN NEUTROPHILS

KW - PLASMA-LEVELS

KW - INTERLEUKIN-8

KW - ANTIBODIES

KW - MARROW

KW - METALLOPROTEINASES

U2 - 10.1089/15258160260090979

DO - 10.1089/15258160260090979

M3 - Article

VL - 11

SP - 513

EP - 521

JO - Journal of hematotherapy & stem cell research

JF - Journal of hematotherapy & stem cell research

SN - 1525-8165

IS - 3

ER -

ID: 13961058