Protein-Templated Dynamic Combinatorial Chemistry: Brief Overview and Experimental Protocol

Hartman, A. M., Gierse, R. M. & Hirsch, A. K. H., 16-Jun-2019, In : European Journal of Organic Chemistry. 2019, 22, p. 3581-3590 10 p.

Research output: Contribution to journalReview articleAcademicpeer-review

Dynamic combinatorial chemistry (DCC) is a powerful tool to identify bioactive compounds. This efficient technique allows the target to select its own binders and circumvents the need for synthesis and biochemical evaluation of all individual derivatives. An ever-increasing number of publications report the use of DCC on biologically relevant target proteins. This minireview complements previous reviews by focusing on the experimental protocol and giving detailed examples of essential steps and factors that need to be considered, such as protein stability, buffer composition and cosolvents.

Original languageEnglish
Pages (from-to)3581-3590
Number of pages10
JournalEuropean Journal of Organic Chemistry
Issue number22
Publication statusPublished - 16-Jun-2019


  • Target-directed dynamic combinatorial chemistry, Protein stability, Hit identification, Medicinal chemistry, Biochemical activity, NUCLEOPHILIC CATALYSIS, HYDRAZONE FORMATION, LARGE NUMBERS, STD NMR, INHIBITORS, LIBRARIES, IDENTIFICATION, LIGAND, GENERATION, OPTIMIZATION

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