Publication

Protein-protein and protein-lipid interactions in domain-assembly: Lessons from giant unilamellar vesicles

Kahya, N., Jul-2010, In : Biochimica et Biophysica Acta-Biomembranes. 1798, 7, p. 1392-1398 7 p.

Research output: Contribution to journalReview articleAcademicpeer-review

Giant Unilamellar Vesicles (GUVs) provide a key model membrane system to study lipid-lipid and lipid-protein interactions, which are relevant to vital cellular processes, by (single-molecule) optical microscopy. Here, we review the work on reconstitution techniques for membrane proteins and other preparation methods for developing GUVs towards most suitable close-to-native membrane systems. Next, we present a few applications of protein-containing GUVs to study domain assembly and protein partitioning into raft-like domains.(C) 2010 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)1392-1398
Number of pages7
JournalBiochimica et Biophysica Acta-Biomembranes
Volume1798
Issue number7
Publication statusPublished - Jul-2010

    Keywords

  • Giant unilamellar vesicle, Lipid raft, Membrane protein reconstitution, Optical microscopy, FLUORESCENCE CORRELATION SPECTROSCOPY, MEMBRANE-PROTEINS, PHYSIOLOGICAL CONDITIONS, ANCHORED PROTEINS, MOLECULAR MOTORS, NATIVE MEMBRANES, CELL-SURFACE, RECONSTITUTION, CHOLESTEROL, BILAYERS

ID: 5112975