Protein-protein and protein-lipid interactions in domain-assembly: Lessons from giant unilamellar vesiclesKahya, N., Jul-2010, In : Biochimica et Biophysica Acta-Biomembranes. 1798, 7, p. 1392-1398 7 p.
Research output: Contribution to journal › Review article › Academic › peer-review
Giant Unilamellar Vesicles (GUVs) provide a key model membrane system to study lipid-lipid and lipid-protein interactions, which are relevant to vital cellular processes, by (single-molecule) optical microscopy. Here, we review the work on reconstitution techniques for membrane proteins and other preparation methods for developing GUVs towards most suitable close-to-native membrane systems. Next, we present a few applications of protein-containing GUVs to study domain assembly and protein partitioning into raft-like domains.(C) 2010 Elsevier B.V. All rights reserved.
|Number of pages||7|
|Journal||Biochimica et Biophysica Acta-Biomembranes|
|Publication status||Published - Jul-2010|
- Giant unilamellar vesicle, Lipid raft, Membrane protein reconstitution, Optical microscopy, FLUORESCENCE CORRELATION SPECTROSCOPY, MEMBRANE-PROTEINS, PHYSIOLOGICAL CONDITIONS, ANCHORED PROTEINS, MOLECULAR MOTORS, NATIVE MEMBRANES, CELL-SURFACE, RECONSTITUTION, CHOLESTEROL, BILAYERS