Publication

Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells

Casciaro, F., Borghesan, M., Beretti, F., Zavatti, M., Bertucci, E., Follo, M. Y., Maraldi, T. & Demaria, M., Oct-2020, In : Mechanisms of Ageing and Development. 191, 10, 12 p., 111328.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Casciaro, F., Borghesan, M., Beretti, F., Zavatti, M., Bertucci, E., Follo, M. Y., Maraldi, T., & Demaria, M. (2020). Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells. Mechanisms of Ageing and Development, 191(10), [111328]. https://doi.org/10.1016/j.mad.2020.111328

Author

Casciaro, Francesca ; Borghesan, Michela ; Beretti, Francesca ; Zavatti, Manuela ; Bertucci, Emma ; Follo, Matilde Yung ; Maraldi, Tullia ; Demaria, Marco. / Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells. In: Mechanisms of Ageing and Development. 2020 ; Vol. 191, No. 10.

Harvard

Casciaro, F, Borghesan, M, Beretti, F, Zavatti, M, Bertucci, E, Follo, MY, Maraldi, T & Demaria, M 2020, 'Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells', Mechanisms of Ageing and Development, vol. 191, no. 10, 111328. https://doi.org/10.1016/j.mad.2020.111328

Standard

Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells. / Casciaro, Francesca; Borghesan, Michela; Beretti, Francesca; Zavatti, Manuela; Bertucci, Emma; Follo, Matilde Yung; Maraldi, Tullia; Demaria, Marco.

In: Mechanisms of Ageing and Development, Vol. 191, No. 10, 111328, 10.2020.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Casciaro F, Borghesan M, Beretti F, Zavatti M, Bertucci E, Follo MY et al. Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells. Mechanisms of Ageing and Development. 2020 Oct;191(10). 111328. https://doi.org/10.1016/j.mad.2020.111328


BibTeX

@article{5d0947b4bbe448d48031578cee6f39b3,
title = "Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells",
abstract = "Human amniotic fluid stem cells (hAFSCs) are an emerging tool in regenerative medicine because they have the ability to differentiate into various lineages and efficiently improve tissue regeneration with no risk of tumorigenesis. Although hAFSCs are easily isolated from the amniotic fluid, their expansion ex vivo is limited by a quick exhaustion which impairs replicative potential and differentiation capacity. In this study, we evaluate various aging features of hAFSCs cultured at different oxygen concentrations. We show that low oxygen (1% O2) extends stemness and proliferative features, and delays induction of senescence-associated markers. Hypoxic hAFSCs activate a metabolic shift and increase resistance to pro-apoptotic stimuli. Moreover, we observe that cells at low oxygen remain capable of osteogenesis for prolonged periods of time, suggesting a more youthful phenotype. Together, these data demonstrate that low oxygen concentrations might improve the generation of functional hAFSCs for therapeutic use by delaying the onset of cellular aging.",
author = "Francesca Casciaro and Michela Borghesan and Francesca Beretti and Manuela Zavatti and Emma Bertucci and Follo, {Matilde Yung} and Tullia Maraldi and Marco Demaria",
note = "Copyright {\textcopyright} 2020 The Author(s). Published by Elsevier B.V. All rights reserved.",
year = "2020",
month = oct,
doi = "10.1016/j.mad.2020.111328",
language = "English",
volume = "191",
journal = "Mechanisms of Ageing and Development",
issn = "0047-6374",
publisher = "ELSEVIER IRELAND LTD",
number = "10",

}

RIS

TY - JOUR

T1 - Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells

AU - Casciaro, Francesca

AU - Borghesan, Michela

AU - Beretti, Francesca

AU - Zavatti, Manuela

AU - Bertucci, Emma

AU - Follo, Matilde Yung

AU - Maraldi, Tullia

AU - Demaria, Marco

N1 - Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

PY - 2020/10

Y1 - 2020/10

N2 - Human amniotic fluid stem cells (hAFSCs) are an emerging tool in regenerative medicine because they have the ability to differentiate into various lineages and efficiently improve tissue regeneration with no risk of tumorigenesis. Although hAFSCs are easily isolated from the amniotic fluid, their expansion ex vivo is limited by a quick exhaustion which impairs replicative potential and differentiation capacity. In this study, we evaluate various aging features of hAFSCs cultured at different oxygen concentrations. We show that low oxygen (1% O2) extends stemness and proliferative features, and delays induction of senescence-associated markers. Hypoxic hAFSCs activate a metabolic shift and increase resistance to pro-apoptotic stimuli. Moreover, we observe that cells at low oxygen remain capable of osteogenesis for prolonged periods of time, suggesting a more youthful phenotype. Together, these data demonstrate that low oxygen concentrations might improve the generation of functional hAFSCs for therapeutic use by delaying the onset of cellular aging.

AB - Human amniotic fluid stem cells (hAFSCs) are an emerging tool in regenerative medicine because they have the ability to differentiate into various lineages and efficiently improve tissue regeneration with no risk of tumorigenesis. Although hAFSCs are easily isolated from the amniotic fluid, their expansion ex vivo is limited by a quick exhaustion which impairs replicative potential and differentiation capacity. In this study, we evaluate various aging features of hAFSCs cultured at different oxygen concentrations. We show that low oxygen (1% O2) extends stemness and proliferative features, and delays induction of senescence-associated markers. Hypoxic hAFSCs activate a metabolic shift and increase resistance to pro-apoptotic stimuli. Moreover, we observe that cells at low oxygen remain capable of osteogenesis for prolonged periods of time, suggesting a more youthful phenotype. Together, these data demonstrate that low oxygen concentrations might improve the generation of functional hAFSCs for therapeutic use by delaying the onset of cellular aging.

U2 - 10.1016/j.mad.2020.111328

DO - 10.1016/j.mad.2020.111328

M3 - Article

C2 - 32800796

VL - 191

JO - Mechanisms of Ageing and Development

JF - Mechanisms of Ageing and Development

SN - 0047-6374

IS - 10

M1 - 111328

ER -

ID: 131683116