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Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells
Casciaro, F., Borghesan, M., Beretti, F., Zavatti, M., Bertucci, E., Follo, M. Y., Maraldi, T. & Demaria, M., Oct-2020, In : Mechanisms of Ageing and Development. 191, 10, 12 p., 111328.Research output: Contribution to journal › Article › Academic › peer-review
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Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells. / Casciaro, Francesca; Borghesan, Michela; Beretti, Francesca; Zavatti, Manuela; Bertucci, Emma; Follo, Matilde Yung; Maraldi, Tullia; Demaria, Marco.
In: Mechanisms of Ageing and Development, Vol. 191, No. 10, 111328, 10.2020.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - Prolonged hypoxia delays aging and preserves functionality of human amniotic fluid stem cells
AU - Casciaro, Francesca
AU - Borghesan, Michela
AU - Beretti, Francesca
AU - Zavatti, Manuela
AU - Bertucci, Emma
AU - Follo, Matilde Yung
AU - Maraldi, Tullia
AU - Demaria, Marco
N1 - Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
PY - 2020/10
Y1 - 2020/10
N2 - Human amniotic fluid stem cells (hAFSCs) are an emerging tool in regenerative medicine because they have the ability to differentiate into various lineages and efficiently improve tissue regeneration with no risk of tumorigenesis. Although hAFSCs are easily isolated from the amniotic fluid, their expansion ex vivo is limited by a quick exhaustion which impairs replicative potential and differentiation capacity. In this study, we evaluate various aging features of hAFSCs cultured at different oxygen concentrations. We show that low oxygen (1% O2) extends stemness and proliferative features, and delays induction of senescence-associated markers. Hypoxic hAFSCs activate a metabolic shift and increase resistance to pro-apoptotic stimuli. Moreover, we observe that cells at low oxygen remain capable of osteogenesis for prolonged periods of time, suggesting a more youthful phenotype. Together, these data demonstrate that low oxygen concentrations might improve the generation of functional hAFSCs for therapeutic use by delaying the onset of cellular aging.
AB - Human amniotic fluid stem cells (hAFSCs) are an emerging tool in regenerative medicine because they have the ability to differentiate into various lineages and efficiently improve tissue regeneration with no risk of tumorigenesis. Although hAFSCs are easily isolated from the amniotic fluid, their expansion ex vivo is limited by a quick exhaustion which impairs replicative potential and differentiation capacity. In this study, we evaluate various aging features of hAFSCs cultured at different oxygen concentrations. We show that low oxygen (1% O2) extends stemness and proliferative features, and delays induction of senescence-associated markers. Hypoxic hAFSCs activate a metabolic shift and increase resistance to pro-apoptotic stimuli. Moreover, we observe that cells at low oxygen remain capable of osteogenesis for prolonged periods of time, suggesting a more youthful phenotype. Together, these data demonstrate that low oxygen concentrations might improve the generation of functional hAFSCs for therapeutic use by delaying the onset of cellular aging.
U2 - 10.1016/j.mad.2020.111328
DO - 10.1016/j.mad.2020.111328
M3 - Article
C2 - 32800796
VL - 191
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 10
M1 - 111328
ER -
ID: 131683116