Publication

Profiling of adhesive-related genes in the freshwater cnidarian Hydra magnipapillata by transcriptomics and proteomics

Rodrigues, M., Ostermann, T., Kremeser, L., Lindner, H., Beisel, C., Berezikov, E., Hobmayer, B. & Ladurner, P., 23-Sep-2016, In : Biofouling. 32, 9, p. 1115-1129 15 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Marcelo Rodrigues
  • Thomas Ostermann
  • Leopold Kremeser
  • Herbert Lindner
  • Christian Beisel
  • Eugene Berezikov
  • Bert Hobmayer
  • Peter Ladurner

The differentiated ectodermal basal disc cells of the freshwater cnidarian Hydra secrete proteinaceous glue to temporarily attach themselves to underwater surfaces. Using transcriptome sequencing and a basal disc-specific RNA-seq combined with in situ hybridisation a highly specific set of candidate adhesive genes was identified. A de novo transcriptome assembly of 55,849 transcripts (>200 bp) was generated using paired-end and single reads from Illumina libraries constructed from different polyp conditions. Differential transcriptomics and spatial gene expression analysis by in situ hybridisation allowed the identification of 40 transcripts exclusively expressed in the ectodermal basal disc cells. Comparisons after mass spectrometry analysis of the adhesive secretion showed a total of 21 transcripts to be basal disc specific and eventually secreted through basal disc cells. This is the first study to survey adhesion-related genes in Hydra. The candidate list presented in this study provides a platform for unravelling the molecular mechanism of underwater adhesion of Hydra.

Original languageEnglish
Pages (from-to)1115-1129
Number of pages15
JournalBiofouling
Volume32
Issue number9
Publication statusPublished - 23-Sep-2016

    Keywords

  • Hydra, basal disc, bioadhesion, transcriptome, differential transcriptome, proteome, RNA-SEQ, COELENTERATE HYDRA, EXPRESSION LEVELS, PROTEIN FAMILIES, CEMENT PROTEINS, PERNA-VIRIDIS, CROSS-LINKING, HOMEOBOX GENE, BASAL DISK, MUSSEL

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