Publication

Primary hepatocytes: Current understanding of the regulation of metabolic enzymes and transporter proteins, and pharmaceutical practice for the use of hepatocytes in metabolism, enzyme induction, transporter, clearance, and hepatotoxicity studies

Hewitt, N. J., Lechon, M. J. G., Houston, J. B., Hallifax, D., Brown, H. S., Maurel, P., Kenna, J. G., Gustavsson, L., Lohmann, C., Skonberg, C., Guillouzo, A., Tuschl, G., Li, A. P., LeCluyse, E., Groothuis, G. M. M. & Hengstler, J. G., 2007, In : Drug metabolism reviews. 39, 1, p. 159-234 76 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Nicola J. Hewitt
  • Maria Jose Gomez Lechon
  • J. Brian Houston
  • David Hallifax
  • Hayley S. Brown
  • Patrick Maurel
  • J. Gerald Kenna
  • Lena Gustavsson
  • Christina Lohmann
  • Christian Skonberg
  • Andre Guillouzo
  • Gregor Tuschl
  • Albert P. Li
  • Edward LeCluyse
  • Geny M. M. Groothuis
  • Jan G. Hengstler

This review brings you up-to-date with the hepatocyte research on: 1) in vitro-in vivo correlations of metabolism and clearance; 2) CYP enzyme induction, regulation, and crosstalk using human hepatocytes and hepatocyte-like cell lines; 3) the function and regulation of hepatic transporters and models used to elucidate their role in drug clearance; 4) mechanisms and examples of idiosyncratic and intrinsic hepatotoxicity; and 5) alternative cell systems to primary human hepatocytes. We also report pharmaceutical perspectives of these topics and compare methods and interpretations for the drug development process.

Original languageEnglish
Pages (from-to)159-234
Number of pages76
JournalDrug metabolism reviews
Volume39
Issue number1
Publication statusPublished - 2007
Event1st Medicon Valley Hepatocyte User Form Symposium - , Denmark
Duration: 26-Jan-200627-Jan-2006

Event

1st Medicon Valley Hepatocyte User Form Symposium

26/01/200627/01/2006

Denmark

Event: Other

    Keywords

  • PREGNANE-X-RECEPTOR, CRYOPRESERVED HUMAN HEPATOCYTES, PRIMARY RAT HEPATOCYTES, HUMAN LIVER-MICROSOMES, IN-VITRO SYSTEMS, NONSTEROIDAL ANTIINFLAMMATORY DRUG, CONSTITUTIVE ANDROSTANE RECEPTOR, COLLAGEN-SANDWICH CONFIGURATION, HUMAN CYTOCHROME P4502B6, ADULT HUMAN HEPATOCYTES

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