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Prevalence and distribution of nucleotide sequences typical for pMEA-like accessory genetic elements in the genus Amycolatopsis

Poele, E. M. T., Habets, M. N., Tan, G. Y. A., Ward, A. C., Goodfellow, M., Bolhuis, H. & Dijkhuizen, L., Aug-2007, In : FEMS microbiology ecology. 61, 2, p. 285-294 10 p.

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  • Prevalence and distribution of nucleotide sequences typical for pMEA-like accessory genetic elements in the genus Amycolatopsis

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DOI

  • Evelien M. te Poele
  • Marrit N. Habets
  • Geok Yuan Annie Tan
  • Alan C. Ward
  • Michael Goodfellow
  • Henk Bolhuis
  • Lubbert Dijkhuizen

The prevalence and distribution of pMEA-like elements in the genus Amycolatopsis a set of 95 recently isolated Amycolatopsis strains and was studied. For this purpose, 16 Amycolatopsis type strains were examined for the presence of two unique pMEA-sequences (repAM and traJ), encoding proteins essential for replication and conjugative transfer. Homologues of repAM and traJ were found in 10 and 26 of 111 investigated strains, respectively, a result which shows that pMEA-like sequences, though not very abundant, can be found in several Amycolatopsis strains. Phylogenetic analysis of the deduced RepAM and TraJ protein sequences revealed clustering with the protein sequences of either pMEA300 or pMEA100. Furthermore, two geographically different populations of pMEA-like elements were distinguished, one originating in Europe and the other in Australia and Asia. Linkage between the distribution of repAM and traJ and the chromosomal identifier, the 16S rRNA gene, indicated that these elements coevolved with their hosts, suggesting that they evolved in an integrated form rather than by horizontal gene transfer of the free replicating form.

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalFEMS microbiology ecology
Volume61
Issue number2
Publication statusPublished - Aug-2007

    Keywords

  • integrative and conjugative element, replication, transfer, geographic distribution, coevolution, Amycolatopsis, SITE-SPECIFIC INTEGRATION, METHYLOTROPHIC ACTINOMYCETE, SP-NOV, STREPTOMYCES-LIVIDANS, SACCHAROPOLYSPORA-ERYTHRAEA, FUNCTIONAL-ANALYSIS, PLASMID PMEA300, DNA-SEQUENCES, METHANOLICA, PSAM2

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