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Preperoxisomal vesicles can form in the absence of Pex3

Knoops, K., Manivannan, S., Cepinska, M. N., Krikken, A. M., Kram, A. M., Veenhuis, M. & van der Klei, I. J., 2014, In : The Journal of Cell Biology. 204, 5, p. 659-668 10 p.

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DOI

We demonstrate that the peroxin Pex3 is not required for the formation of peroxisomal membrane structures in yeast pex3 mutant cells. Notably, pex3 mutant cells already contain reticular and vesicular structures that harbor key proteins of the peroxisomal receptor docking complex-Pex13 and Pex14-as well as the matrix proteins Pex8 and alcohol oxidase. Other peroxisomal membrane proteins in these cells are unstable and transiently localized to the cytosol (Pex10, Pmp47) or endoplasmic reticulum (Pex11). These reticular and vesicular structures are more abundant in cells of a pex3 atgl double deletion strain, as the absence of Pex3 may render them susceptible to autophagic degradation, which is blocked in this double mutant. Contrary to earlier suggestions, peroxisomes are not formed de novo from the endoplasmic reticulum when the PEX3 gene is reintroduced in pex3 cells. Instead, we find that reintroduced Pex3 sorts to the preperoxisomal structures in pex3 cells, after which these structures mature into normal peroxisomes.

Original languageEnglish
Pages (from-to)659-668
Number of pages10
JournalThe Journal of Cell Biology
Volume204
Issue number5
Publication statusPublished - 2014

    Keywords

  • PEROXISOMAL MEMBRANE-PROTEINS, HANSENULA-POLYMORPHA, ENDOPLASMIC-RETICULUM, BIOGENESIS, REQUIRES, IMPORT, REINTRODUCTION, PROLIFERATION, DEGRADATION, DIVISION

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