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Preparation of Monodisperse Giant Unilamellar Anchored Vesicles Using Micropatterned Hydrogel Substrates

Schultze, J., Vagias, A., Ye, L., Prantl, E., Breising, V., Best, A., Koynov, K., Marques, C. M. & Butt, H-J., 31-May-2019, In : ACS Omega. 4, 5, p. 9393-9399 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Jennifer Schultze
  • Apostolos Vagias
  • Lijun Ye
  • Ephraim Prantl
  • Valentina Breising
  • Andreas Best
  • Kaloian Koynov
  • Carlos M. Marques
  • Hans-Juergen Butt

Giant unilamellar vesicles (GUVs) are model membrane systems consisting of a single lipid bilayer separating an inner lumen from the outer solution, with dimensions comparable to that of eukaryotic cells. The importance of these biomimetic systems has recently grown with the development of easy and safe methods to assemble GUVs from complex biorelevant compositions. However, size and position control is still a key challenge for GUV formation and manipulation. Here, a gel-assisted formation method is introduced, able to produce arrays of giant unilamellar anchored vesicles (GUAVs) with a predetermined narrow size distribution. The approach based on micropatterned gel substrates of cross-linked poly(N-isopropylacrylamide) allows performing parallel measurements on thousands of immobile unilamellar vesicles. Such power and flexibility will respond to the growing need for developing platforms of biomimetic constructs from cell-sized single bilayers.

Original languageEnglish
Pages (from-to)9393-9399
Number of pages7
JournalACS Omega
Volume4
Issue number5
Publication statusPublished - 31-May-2019

    Keywords

  • FLUORESCENCE CORRELATION SPECTROSCOPY, LIPID-BILAYERS, DIFFUSION, OXIDATION, DYNAMICS, LAYERS, FILMS, SIZE

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