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Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach to Detect an Orthopaedic Implant Infection

Wang, Y., Thompson, J. M., Ashbaugh, A. G., Khodakivskyi, P., Budin, G., Sinisi, R., Heinmiller, A., van Oosten, M., van Dijl, J. M., van Dam, G. M., Francis, K. P., Bernthal, N. M., Dubikovskaya, E. A. & Miller, L. S., 2017, In : The Journal of the American Academy of Orthopaedic Surgeons. 25, p. S7-S12 6 p.

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  • Preclinical Evaluation of Photoacoustic Imaging as a Novel Noninvasive Approach

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DOI

Introduction: Diagnosing-prosthetic joint infection (PJI) poses significant challenges, and current modalities are fraught with low sensitivity and/or potential morbidity. Photoacoustic imaging (PAI) is a novel ultrasound-based modality with potential for diagnosing PJI safely and noninvasively.

Materials: In an established preclinical mouse model of bioluminescent Staphylococcus aureus PJI, fluorescent indocyanine green (ICG) was conjugated to beta-cyclodextrin (CDX-ICG) or teicoplanin (Teic-ICG) and injected intravenously for 1 week postoperatively. Daily fluorescent imaging and PAI were used to localize and quantify tracer signals.

Results were analyzed using 2-way analysis of variance. Results: Fluorescence clearly localized to the site of infection and was significantly higher with Teic-ICG compared with CDX-ICG (P = 0.046) and ICG alone (P = 0.0087). With PAI, the photoacoustic signal per volumetric analysis was substantially higher and better visualized with Teic-ICG compared with CDX-ICG and ICG alone, and colocalized well with bioluminescence and fluorescence imaging.

Conclusion: Photoacoustic imaging successfully localized PJI in this proof-of concept study and demonstrates potential for clinical translation in orthopaedics.

Original languageEnglish
Pages (from-to)S7-S12
Number of pages6
JournalThe Journal of the American Academy of Orthopaedic Surgeons
Volume25
Publication statusPublished - 2017

    Keywords

  • PROSTHETIC JOINT INFECTION, FDG-PET, ULTRASOUND, MARKERS, HIP

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