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PODOCYTE EXPRESSION OF MHC CLASS-I AND CLASS-II AND INTERCELLULAR-ADHESION MOLECULE-1 (ICAM-1) IN EXPERIMENTAL PAUCI-IMMUNE CRESCENTIC GLOMERULONEPHRITIS

Coers, W., BROUWER, E., Vos, J. TWM., CHAND, A., Huitema, S., Heeringa, P., Kallenberg, C. & Weening, J. J., Nov-1994, In : Clinical and Experimental Immunology. 98, 2, p. 279 - 286 8 p.

Research output: Contribution to journalArticleAcademicpeer-review

We examined immunopathological changes of podocytes in vivo which, based on in vitro studies, are thought to be relevant for the pathogenesis of renal diseases. We investigated the alterations of podocytes in local inflammation in a recently developed model of pauci-immune necrotizing crescentic glomerulonephritis (NCGN) in the rat. Frozen and plastic embedded kidney sections at different time points of the disease were incubated with antibodies directed to MHC class I, MHC class II, ICAM-1 and to relevant cytokines. Strong glomerular expression of MHC class I, II and ICAM-1 was found within 4 days, and plastic embedded sections clearly demonstrated increased cell membrane staining of podocytes. Increased glomerular interferon-gamma (IFN-gamma) was detected within 24 h of induction of NCGN, and IL-1 beta and tumour necrosis factor-alpha (TNF-alpha) were found from day 4. The potency of these cytokines to induce adhesion molecules on podocytes was investigated on rat glomerular epithelial cells in vitro. By using FACS analysis and electron microscopical techniques, we found that the in vivo expression of MHC class I, II and ICAM-1 by podocytes could in vitro be simulated by IFN-gamma. IFN-alpha weakly induced MHC class I, while IL-1 beta and TNF-alpha were ineffective. We hypothesize that podocytes in this in vivo model are important to maintain the local inflammatory process in the glomerulus by expression of relevant adhesion molecules and MHC molecules upon stimulation with specific cytokines.

Original languageEnglish
Pages (from-to)279 - 286
Number of pages8
JournalClinical and Experimental Immunology
Volume98
Issue number2
Publication statusPublished - Nov-1994

    Keywords

  • GLOMERULAR VISCERAL EPITHELIAL CELLS, MHC, INTERCELLULAR ADHESION MOLECULE-1, INTERFERON-ALPHA, INTERFERON-GAMMA, GLOMERULAR EPITHELIAL-CELLS, INTERFERON-GAMMA, ANTIGEN PRESENTATION, MESANGIAL CELLS, RAT-KIDNEY, ALPHA-BETA, ANTIBODIES, CYTOKINES, MODULATION

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