Publication

Plasma hemopexin activity in pregnancy and preeclampsia

Bakker, W. W., Donker, R. B., Timmer, A., van Pampus, M. G., van Son, W. J., Aarnoudse, J. G., van Goor, H., Niezen-Koning, K. E., Navis, G., Borghuis, T., Jongman, R. M. & Faas, M. M., 2007, In : HYPERTENSION IN PREGNANCY. 26, 2, p. 227-239 13 p.

Research output: Contribution to journalArticleAcademicpeer-review

Objective: Plasma hemopexin activity, associated with increased vascular permeability, was evaluated in healthy pregnant and non-pregnant women and in pre-eclamptic women. Methods: Hemopexin activity and the hemopexin inhibitor, extracellular ATP, were assayed in plasma from pregnant (n=10), preeclamptic (n=9), and non-pregnant women (n=10) using standard methods. Abdominal fascia tissue fragments from preeclamptic and pregnant women were immunohistochemically stained for vascular ectoapyrase or ecto-5'nucleotidase. Results: The data show significantly enhanced Hx activity exclusively in plasma from pregnant women and significantly enhanced plasma ATP in pre-eclamptic women compared with the other groups. Dephosphorylation of preeclamptic plasma resulted in reactivation of Hx activity. Fascia tissue-samples from preeclamptic women showed reduced ecto-apyrase activity and enhanced ecto-5'nucleotidase activity compared to pregnant women. Conclusion: Enhanced hemopexin activity may be associated with normal pregnancy, but not with preeclampsia. Decreased hemopexin in pre-eclamptic patients may be due to enhanced plasma ATP, which is possibly promoted by diminished activity of vascular ecto-apyrase.

Original languageEnglish
Pages (from-to)227-239
Number of pages13
JournalHYPERTENSION IN PREGNANCY
Volume26
Issue number2
Publication statusPublished - 2007

    Keywords

  • hemopexin, pregnancy, preeclampsia, ADENINE-NUCLEOTIDES, HUMAN PLACENTA, ADENOSINE, CIRCULATION, MEMBRANES, INFUSION, PURINES, ATPASE

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