Publication

Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species

Buitenhuis, E. T., Baar, H. J. W. D. & Veldhuis, M. J. W., 1999, In : Journal of Phycology. 35, 5, p. 949-959 11 p.

Research output: Contribution to journalArticleAcademic

APA

Buitenhuis, E. T., Baar, H. J. W. D., & Veldhuis, M. J. W. (1999). Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species. Journal of Phycology, 35(5), 949-959. https://doi.org/10.1046/j.1529-8817.1999.3550949.x

Author

Buitenhuis, Erik T. ; Baar, Hein J.W. de ; Veldhuis, Marcel J.W. / Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species. In: Journal of Phycology. 1999 ; Vol. 35, No. 5. pp. 949-959.

Harvard

Buitenhuis, ET, Baar, HJWD & Veldhuis, MJW 1999, 'Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species', Journal of Phycology, vol. 35, no. 5, pp. 949-959. https://doi.org/10.1046/j.1529-8817.1999.3550949.x

Standard

Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species. / Buitenhuis, Erik T.; Baar, Hein J.W. de; Veldhuis, Marcel J.W.

In: Journal of Phycology, Vol. 35, No. 5, 1999, p. 949-959.

Research output: Contribution to journalArticleAcademic

Vancouver

Buitenhuis ET, Baar HJWD, Veldhuis MJW. Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species. Journal of Phycology. 1999;35(5):949-959. https://doi.org/10.1046/j.1529-8817.1999.3550949.x


BibTeX

@article{5cb8f469613a4251a85cf503172ef691,
title = "Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species",
abstract = "To test the possibility of inorganic carbon limitation of the marine unicellular alga Emiliania huxleyi (Lohmann) Hay and Mohler, its carbon acquisition was measured as a function of the different chemical species of inorganic carbon present in the medium. Because these different species are interdependent and covary in any experiment in which the speciation is changed, a set of experiments was performed to produce a multidimensional carbon uptake scheme for photosynthesis and calcification. This scheme shows that CO2 that is used for photosynthesis comes from two sources. The CO2 in seawater supports a modest rate of photosynthesis. The HCO3- is the major substrate for photosynthesis by intracellular production of CO2 (HCO3- + H+ → CO2 + H2O → CH2O + O2). This use of HCO3- is possible because of the simultaneous calcification using a second HCO3- , which provides the required proton (HCO3- + Ca2+ → CaCO3 + H+). The HCO3- is the only substrate for calcification. By distinguishing the two sources of CO2 used in photosynthesis, it was shown that E. huxleyi has a K½ for external CO2 of ‘‘only’’ 1.9 ± 0.5 µM (and a Vmax of 2.4 ± 0.1 pmol·cell-1·d-1). Thus, in seawater that is in equilibrium with the atmosphere ([CO2] = 14 µM, [HCO3-] = 1920 µM, at fCO2 = 360 µatm, pH = 8, T = 15° C), photosynthesis is 90{\%} saturated with external CO2. Under the same conditions, the rate of photosynthesis is doubled by the calcification route of CO2 supply (from 2.1 to 4.5 pmol·cell-1·d-1). However, photosynthesis is not fully saturated, as calcification has a K½ for HCO3- of 3256 ± 1402 µM and a Vmax of 6.4 ± 1.8 pmol·cell-1·d-1. The H+ that is produced during calcification is used with an efficiency of 0.97 ± 0.08, leading to the conclusion that it is used intracellularly. A maximum efficiency of 0.88 can be expected, as NO3- uptake generates a H+ sink (OH- source) for the cell. The success of E. huxleyi as a coccolithophorid may be related to the efficient coupling between H+ generation in calcification and CO2 fixation in photosynthesis.",
keywords = "photosynthetic carbon fixation, pH, HCO3- (bicarbonate), Haptophyta, Emiliania huxleyi, dissolved inorganic carbon system, CO2 (carbon dioxide), coccolithophorid, calcification rate",
author = "Buitenhuis, {Erik T.} and Baar, {Hein J.W. de} and Veldhuis, {Marcel J.W.}",
note = "Relation: https://www.rug.nl/research/cees/ Rights: University of Groningen, Centre for Ecological and Evolutionary Studies",
year = "1999",
doi = "10.1046/j.1529-8817.1999.3550949.x",
language = "English",
volume = "35",
pages = "949--959",
journal = "Journal of Phycology",
issn = "0022-3646",
publisher = "Wiley",
number = "5",

}

RIS

TY - JOUR

T1 - Photosynthesis and Calcification by Emiliania huxleyi (Prymnesiophyceae) as a Function of Inorganic Carbon Species

AU - Buitenhuis, Erik T.

AU - Baar, Hein J.W. de

AU - Veldhuis, Marcel J.W.

N1 - Relation: https://www.rug.nl/research/cees/ Rights: University of Groningen, Centre for Ecological and Evolutionary Studies

PY - 1999

Y1 - 1999

N2 - To test the possibility of inorganic carbon limitation of the marine unicellular alga Emiliania huxleyi (Lohmann) Hay and Mohler, its carbon acquisition was measured as a function of the different chemical species of inorganic carbon present in the medium. Because these different species are interdependent and covary in any experiment in which the speciation is changed, a set of experiments was performed to produce a multidimensional carbon uptake scheme for photosynthesis and calcification. This scheme shows that CO2 that is used for photosynthesis comes from two sources. The CO2 in seawater supports a modest rate of photosynthesis. The HCO3- is the major substrate for photosynthesis by intracellular production of CO2 (HCO3- + H+ → CO2 + H2O → CH2O + O2). This use of HCO3- is possible because of the simultaneous calcification using a second HCO3- , which provides the required proton (HCO3- + Ca2+ → CaCO3 + H+). The HCO3- is the only substrate for calcification. By distinguishing the two sources of CO2 used in photosynthesis, it was shown that E. huxleyi has a K½ for external CO2 of ‘‘only’’ 1.9 ± 0.5 µM (and a Vmax of 2.4 ± 0.1 pmol·cell-1·d-1). Thus, in seawater that is in equilibrium with the atmosphere ([CO2] = 14 µM, [HCO3-] = 1920 µM, at fCO2 = 360 µatm, pH = 8, T = 15° C), photosynthesis is 90% saturated with external CO2. Under the same conditions, the rate of photosynthesis is doubled by the calcification route of CO2 supply (from 2.1 to 4.5 pmol·cell-1·d-1). However, photosynthesis is not fully saturated, as calcification has a K½ for HCO3- of 3256 ± 1402 µM and a Vmax of 6.4 ± 1.8 pmol·cell-1·d-1. The H+ that is produced during calcification is used with an efficiency of 0.97 ± 0.08, leading to the conclusion that it is used intracellularly. A maximum efficiency of 0.88 can be expected, as NO3- uptake generates a H+ sink (OH- source) for the cell. The success of E. huxleyi as a coccolithophorid may be related to the efficient coupling between H+ generation in calcification and CO2 fixation in photosynthesis.

AB - To test the possibility of inorganic carbon limitation of the marine unicellular alga Emiliania huxleyi (Lohmann) Hay and Mohler, its carbon acquisition was measured as a function of the different chemical species of inorganic carbon present in the medium. Because these different species are interdependent and covary in any experiment in which the speciation is changed, a set of experiments was performed to produce a multidimensional carbon uptake scheme for photosynthesis and calcification. This scheme shows that CO2 that is used for photosynthesis comes from two sources. The CO2 in seawater supports a modest rate of photosynthesis. The HCO3- is the major substrate for photosynthesis by intracellular production of CO2 (HCO3- + H+ → CO2 + H2O → CH2O + O2). This use of HCO3- is possible because of the simultaneous calcification using a second HCO3- , which provides the required proton (HCO3- + Ca2+ → CaCO3 + H+). The HCO3- is the only substrate for calcification. By distinguishing the two sources of CO2 used in photosynthesis, it was shown that E. huxleyi has a K½ for external CO2 of ‘‘only’’ 1.9 ± 0.5 µM (and a Vmax of 2.4 ± 0.1 pmol·cell-1·d-1). Thus, in seawater that is in equilibrium with the atmosphere ([CO2] = 14 µM, [HCO3-] = 1920 µM, at fCO2 = 360 µatm, pH = 8, T = 15° C), photosynthesis is 90% saturated with external CO2. Under the same conditions, the rate of photosynthesis is doubled by the calcification route of CO2 supply (from 2.1 to 4.5 pmol·cell-1·d-1). However, photosynthesis is not fully saturated, as calcification has a K½ for HCO3- of 3256 ± 1402 µM and a Vmax of 6.4 ± 1.8 pmol·cell-1·d-1. The H+ that is produced during calcification is used with an efficiency of 0.97 ± 0.08, leading to the conclusion that it is used intracellularly. A maximum efficiency of 0.88 can be expected, as NO3- uptake generates a H+ sink (OH- source) for the cell. The success of E. huxleyi as a coccolithophorid may be related to the efficient coupling between H+ generation in calcification and CO2 fixation in photosynthesis.

KW - photosynthetic carbon fixation

KW - pH

KW - HCO3- (bicarbonate)

KW - Haptophyta

KW - Emiliania huxleyi

KW - dissolved inorganic carbon system

KW - CO2 (carbon dioxide)

KW - coccolithophorid

KW - calcification rate

U2 - 10.1046/j.1529-8817.1999.3550949.x

DO - 10.1046/j.1529-8817.1999.3550949.x

M3 - Article

VL - 35

SP - 949

EP - 959

JO - Journal of Phycology

JF - Journal of Phycology

SN - 0022-3646

IS - 5

ER -

ID: 3170183