Publication

Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha

Thomas, A. S., Krikken, A. M., van der Klei, I. J. & Williams, C. P., 2015, In : Scientific Reports. 5, p. 1-11 11 p., 11493.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Thomas, A. S., Krikken, A. M., van der Klei, I. J., & Williams, C. P. (2015). Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha. Scientific Reports, 5, 1-11. [11493]. https://doi.org/10.1038/srep11493

Author

Thomas, Ann S ; Krikken, Arjen M ; van der Klei, Ida J ; Williams, Chris P. / Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha. In: Scientific Reports. 2015 ; Vol. 5. pp. 1-11.

Harvard

Thomas, AS, Krikken, AM, van der Klei, IJ & Williams, CP 2015, 'Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha', Scientific Reports, vol. 5, 11493, pp. 1-11. https://doi.org/10.1038/srep11493

Standard

Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha. / Thomas, Ann S; Krikken, Arjen M; van der Klei, Ida J; Williams, Chris P.

In: Scientific Reports, Vol. 5, 11493, 2015, p. 1-11.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Thomas AS, Krikken AM, van der Klei IJ, Williams CP. Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha. Scientific Reports. 2015;5:1-11. 11493. https://doi.org/10.1038/srep11493


BibTeX

@article{fb619229b11240f9af0cc305b5f752f8,
title = "Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha",
abstract = "Pex11p plays a crucial role in peroxisomal fission. Studies in Saccharomyces cerevisiae and Pichia pastoris indicated that Pex11p is activated by phosphorylation, which results in enhanced peroxisome proliferation. In S. cerevisiae but not in P. pastoris, Pex11p phosphorylation was shown to regulate the protein's trafficking to peroxisomes. However, phosphorylation of PpPex11p was proposed to influence its interaction with Fis1p, another component of the organellar fission machinery. Here, we have examined the role of Pex11p phosphorylation in the yeast Hansenula polymorpha. Employing mass spectrometry, we demonstrate that HpPex11p is also phosphorylated on a Serine residue present at a similar position to that of ScPex11p and PpPex11p. Furthermore, through the use of mutants designed to mimic both phosphorylated and unphosphorylated forms of HpPex11p, we have investigated the role of this post-translational modification. Our data demonstrate that mutations to the phosphorylation site do not disturb the function of Pex11p in peroxisomal fission, nor do they alter the localization of Pex11p. Also, no effect on peroxisome inheritance was observed. Taken together, these data lead us to conclude that peroxisomal fission in H. polymorpha is not modulated by phosphorylation of Pex11p.",
author = "Thomas, {Ann S} and Krikken, {Arjen M} and {van der Klei}, {Ida J} and Williams, {Chris P}",
year = "2015",
doi = "10.1038/srep11493",
language = "English",
volume = "5",
pages = "1--11",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Phosphorylation of Pex11p does not regulate peroxisomal fission in the yeast Hansenula polymorpha

AU - Thomas, Ann S

AU - Krikken, Arjen M

AU - van der Klei, Ida J

AU - Williams, Chris P

PY - 2015

Y1 - 2015

N2 - Pex11p plays a crucial role in peroxisomal fission. Studies in Saccharomyces cerevisiae and Pichia pastoris indicated that Pex11p is activated by phosphorylation, which results in enhanced peroxisome proliferation. In S. cerevisiae but not in P. pastoris, Pex11p phosphorylation was shown to regulate the protein's trafficking to peroxisomes. However, phosphorylation of PpPex11p was proposed to influence its interaction with Fis1p, another component of the organellar fission machinery. Here, we have examined the role of Pex11p phosphorylation in the yeast Hansenula polymorpha. Employing mass spectrometry, we demonstrate that HpPex11p is also phosphorylated on a Serine residue present at a similar position to that of ScPex11p and PpPex11p. Furthermore, through the use of mutants designed to mimic both phosphorylated and unphosphorylated forms of HpPex11p, we have investigated the role of this post-translational modification. Our data demonstrate that mutations to the phosphorylation site do not disturb the function of Pex11p in peroxisomal fission, nor do they alter the localization of Pex11p. Also, no effect on peroxisome inheritance was observed. Taken together, these data lead us to conclude that peroxisomal fission in H. polymorpha is not modulated by phosphorylation of Pex11p.

AB - Pex11p plays a crucial role in peroxisomal fission. Studies in Saccharomyces cerevisiae and Pichia pastoris indicated that Pex11p is activated by phosphorylation, which results in enhanced peroxisome proliferation. In S. cerevisiae but not in P. pastoris, Pex11p phosphorylation was shown to regulate the protein's trafficking to peroxisomes. However, phosphorylation of PpPex11p was proposed to influence its interaction with Fis1p, another component of the organellar fission machinery. Here, we have examined the role of Pex11p phosphorylation in the yeast Hansenula polymorpha. Employing mass spectrometry, we demonstrate that HpPex11p is also phosphorylated on a Serine residue present at a similar position to that of ScPex11p and PpPex11p. Furthermore, through the use of mutants designed to mimic both phosphorylated and unphosphorylated forms of HpPex11p, we have investigated the role of this post-translational modification. Our data demonstrate that mutations to the phosphorylation site do not disturb the function of Pex11p in peroxisomal fission, nor do they alter the localization of Pex11p. Also, no effect on peroxisome inheritance was observed. Taken together, these data lead us to conclude that peroxisomal fission in H. polymorpha is not modulated by phosphorylation of Pex11p.

U2 - 10.1038/srep11493

DO - 10.1038/srep11493

M3 - Article

VL - 5

SP - 1

EP - 11

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 11493

ER -

ID: 20877105