Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteinsBlaauw, M., Knol, JC., Kortholt, A., Roelofs, J., Ruchira, N. V., Postma, M., Visser, AJWG., Van Haastert, PJM., Knol, J. C. & Visser, A. J. W. G., 1-Oct-2003, In : EMBO Journal. 22, 19, p. 5047-5057 11 p.
Research output: Contribution to journal › Article › Academic › peer-review
Retinal phosducin is known to sequester transducin Gbetagamma, thereby modulating transducin activity. Phos ducin is a member of a family of phosducin-like proteins (PhLP) found in eukaryotes. Phylogeny of 33 phosducin-like proteins from metazoa, plants and lower eukaryotes identified three distinct groups named phosducin-I-III. We discovered three phlp genes in Dictyostelium, each encoding a phosducin-like protein of a different group. Disruption of the phlp1 gene strongly impaired G-protein signalling, apparently due to mislocalization of Gbetagamma in phlp1-null cells. GFP-Gbeta and GFP-Ggamma are membrane associated in wild-type cells, but cytosolic in phlp1-null cells. Phlp2 disruption is lethal due to a synchronous collapse of the cells after 16-17 cell divisions. Phlp3 disruptants show no abnormal phenotype. These results establish a role for phosducin-like proteins in facilitating folding, localization or function of proteins, in addition to modulating G-protein signalling.
|Number of pages||11|
|Publication status||Published - 1-Oct-2003|
- chaperone, Dictyostelium, G protein, signal transduction, phosducin, G-BETA-GAMMA, HETEROTRIMERIC G-PROTEINS, SUBUNIT FUNCTION, REGULATOR, COMPLEX, CHEMOTAXIS, TRANSDUCIN, CELLS, PHLP, GRADIENTS