Publication

PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis: Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB

Faria, D. D. P., Copray, S., Sijbesma, J. W. A., Willemsen, A. T. M., Buchpiguel, C. A., Dierckx, R. A. J. O. & de Vries, E. F. J., May-2014, In : European Journal of Nuclear Medicine and Molecular Imaging. 41, 5, p. 995-1003 9 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Faria, D. D. P., Copray, S., Sijbesma, J. W. A., Willemsen, A. T. M., Buchpiguel, C. A., Dierckx, R. A. J. O., & de Vries, E. F. J. (2014). PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis: Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB. European Journal of Nuclear Medicine and Molecular Imaging, 41(5), 995-1003. https://doi.org/10.1007/s00259-013-2682-6

Author

Faria, Daniele de Paula ; Copray, Sjef ; Sijbesma, Jurgen W. A. ; Willemsen, Antoon T. M. ; Buchpiguel, Carlos A. ; Dierckx, Rudi A. J. O. ; de Vries, Erik F. J. / PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis : Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB. In: European Journal of Nuclear Medicine and Molecular Imaging. 2014 ; Vol. 41, No. 5. pp. 995-1003.

Harvard

Faria, DDP, Copray, S, Sijbesma, JWA, Willemsen, ATM, Buchpiguel, CA, Dierckx, RAJO & de Vries, EFJ 2014, 'PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis: Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB', European Journal of Nuclear Medicine and Molecular Imaging, vol. 41, no. 5, pp. 995-1003. https://doi.org/10.1007/s00259-013-2682-6

Standard

PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis : Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB. / Faria, Daniele de Paula; Copray, Sjef; Sijbesma, Jurgen W. A.; Willemsen, Antoon T. M.; Buchpiguel, Carlos A.; Dierckx, Rudi A. J. O.; de Vries, Erik F. J.

In: European Journal of Nuclear Medicine and Molecular Imaging, Vol. 41, No. 5, 05.2014, p. 995-1003.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Faria DDP, Copray S, Sijbesma JWA, Willemsen ATM, Buchpiguel CA, Dierckx RAJO et al. PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis: Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB. European Journal of Nuclear Medicine and Molecular Imaging. 2014 May;41(5):995-1003. https://doi.org/10.1007/s00259-013-2682-6


BibTeX

@article{3f2f5e5a71e846c887ae6c754a7415f3,
title = "PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis: Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB",
abstract = "PURPOSE: In this study, we compared the ability of [(11)C]CIC, [(11)C]MeDAS and [(11)C]PIB to reveal temporal changes in myelin content in focal lesions in the lysolecithin rat model of multiple sclerosis. Pharmacokinetic modelling was performed to determine the best method to quantify tracer uptake.METHODS: Sprague-Dawley rats were stereotactically injected with either 1 {\%} lysolecithin or saline into the corpus callosum and striatum of the right brain hemisphere. Dynamic PET imaging with simultaneous arterial blood sampling was performed 7 days after saline injection (control group), 7 days after lysolecithin injection (demyelination group) and 4 weeks after lysolecithin injection (remyelination group).RESULTS: The kinetics of [(11)C]CIC, [(11)C]MeDAS and [(11)C]PIB was best fitted by Logan graphical analysis, suggesting that tracer binding is reversible. Compartment modelling revealed that all tracers were fitted best with the reversible two-tissue compartment model. Tracer uptake and distribution volume in lesions were in agreement with myelin status. However, the slow kinetics and homogeneous brain uptake of [(11)C]CIC make this tracer less suitable for in vivo PET imaging. [(11)C]PIB showed good uptake in the white matter in the cerebrum, but [(11)C]PIB uptake in the cerebellum was low, despite high myelin density in this region. [(11)C]MeDAS distribution correlated well with myelin density in different brain regions.CONCLUSION: This study showed that PET imaging of demyelination and remyelination processes in focal lesions is feasible. Our comparison of three myelin tracers showed that [(11)C]MeDAS has more favourable properties for quantitative PET imaging of demyelinated and remyelinated lesions throughout the CNS than [(11)C]CIC and [(11)C]PIB.",
keywords = "Demyelination, Remyelination, PET imaging, Myelin, Multiple sclerosis, IN-VIVO QUANTIFICATION, PITTSBURGH COMPOUND-B, ALZHEIMERS-DISEASE, LYSOPHOSPHATIDYL CHOLINE, WHITE-MATTER, SPINAL-CORD, MYELIN, INJECTIONS, BINDING, REPAIR",
author = "Faria, {Daniele de Paula} and Sjef Copray and Sijbesma, {Jurgen W. A.} and Willemsen, {Antoon T. M.} and Buchpiguel, {Carlos A.} and Dierckx, {Rudi A. J. O.} and {de Vries}, {Erik F. J.}",
year = "2014",
month = "5",
doi = "10.1007/s00259-013-2682-6",
language = "English",
volume = "41",
pages = "995--1003",
journal = "European Journal of Nuclear Medicine and Molecular Imaging",
issn = "1619-7070",
publisher = "SPRINGER",
number = "5",

}

RIS

TY - JOUR

T1 - PET imaging of focal demyelination and remyelination in a rat model of multiple sclerosis

T2 - Comparison of [C-11]MeDAS, [C-11]CIC and [C-11]PIB

AU - Faria, Daniele de Paula

AU - Copray, Sjef

AU - Sijbesma, Jurgen W. A.

AU - Willemsen, Antoon T. M.

AU - Buchpiguel, Carlos A.

AU - Dierckx, Rudi A. J. O.

AU - de Vries, Erik F. J.

PY - 2014/5

Y1 - 2014/5

N2 - PURPOSE: In this study, we compared the ability of [(11)C]CIC, [(11)C]MeDAS and [(11)C]PIB to reveal temporal changes in myelin content in focal lesions in the lysolecithin rat model of multiple sclerosis. Pharmacokinetic modelling was performed to determine the best method to quantify tracer uptake.METHODS: Sprague-Dawley rats were stereotactically injected with either 1 % lysolecithin or saline into the corpus callosum and striatum of the right brain hemisphere. Dynamic PET imaging with simultaneous arterial blood sampling was performed 7 days after saline injection (control group), 7 days after lysolecithin injection (demyelination group) and 4 weeks after lysolecithin injection (remyelination group).RESULTS: The kinetics of [(11)C]CIC, [(11)C]MeDAS and [(11)C]PIB was best fitted by Logan graphical analysis, suggesting that tracer binding is reversible. Compartment modelling revealed that all tracers were fitted best with the reversible two-tissue compartment model. Tracer uptake and distribution volume in lesions were in agreement with myelin status. However, the slow kinetics and homogeneous brain uptake of [(11)C]CIC make this tracer less suitable for in vivo PET imaging. [(11)C]PIB showed good uptake in the white matter in the cerebrum, but [(11)C]PIB uptake in the cerebellum was low, despite high myelin density in this region. [(11)C]MeDAS distribution correlated well with myelin density in different brain regions.CONCLUSION: This study showed that PET imaging of demyelination and remyelination processes in focal lesions is feasible. Our comparison of three myelin tracers showed that [(11)C]MeDAS has more favourable properties for quantitative PET imaging of demyelinated and remyelinated lesions throughout the CNS than [(11)C]CIC and [(11)C]PIB.

AB - PURPOSE: In this study, we compared the ability of [(11)C]CIC, [(11)C]MeDAS and [(11)C]PIB to reveal temporal changes in myelin content in focal lesions in the lysolecithin rat model of multiple sclerosis. Pharmacokinetic modelling was performed to determine the best method to quantify tracer uptake.METHODS: Sprague-Dawley rats were stereotactically injected with either 1 % lysolecithin or saline into the corpus callosum and striatum of the right brain hemisphere. Dynamic PET imaging with simultaneous arterial blood sampling was performed 7 days after saline injection (control group), 7 days after lysolecithin injection (demyelination group) and 4 weeks after lysolecithin injection (remyelination group).RESULTS: The kinetics of [(11)C]CIC, [(11)C]MeDAS and [(11)C]PIB was best fitted by Logan graphical analysis, suggesting that tracer binding is reversible. Compartment modelling revealed that all tracers were fitted best with the reversible two-tissue compartment model. Tracer uptake and distribution volume in lesions were in agreement with myelin status. However, the slow kinetics and homogeneous brain uptake of [(11)C]CIC make this tracer less suitable for in vivo PET imaging. [(11)C]PIB showed good uptake in the white matter in the cerebrum, but [(11)C]PIB uptake in the cerebellum was low, despite high myelin density in this region. [(11)C]MeDAS distribution correlated well with myelin density in different brain regions.CONCLUSION: This study showed that PET imaging of demyelination and remyelination processes in focal lesions is feasible. Our comparison of three myelin tracers showed that [(11)C]MeDAS has more favourable properties for quantitative PET imaging of demyelinated and remyelinated lesions throughout the CNS than [(11)C]CIC and [(11)C]PIB.

KW - Demyelination

KW - Remyelination

KW - PET imaging

KW - Myelin

KW - Multiple sclerosis

KW - IN-VIVO QUANTIFICATION

KW - PITTSBURGH COMPOUND-B

KW - ALZHEIMERS-DISEASE

KW - LYSOPHOSPHATIDYL CHOLINE

KW - WHITE-MATTER

KW - SPINAL-CORD

KW - MYELIN

KW - INJECTIONS

KW - BINDING

KW - REPAIR

U2 - 10.1007/s00259-013-2682-6

DO - 10.1007/s00259-013-2682-6

M3 - Article

VL - 41

SP - 995

EP - 1003

JO - European Journal of Nuclear Medicine and Molecular Imaging

JF - European Journal of Nuclear Medicine and Molecular Imaging

SN - 1619-7070

IS - 5

ER -

ID: 14256124