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PET Imaging of Disease Progression and Treatment Effects in the Experimental Autoimmune Encephalomyelitis Rat Model

Faria, D. D. P., Vlaming, M. L. H., Copray, S. C. V. M., Tielen, F., Anthonijsz, H. J. A., Sijbesma, J. W. A., Buchpiguel, C. A., Dierckx, R. A. J. O., van der Hoorn, J. W. A. & de Vries, E. F. J., Aug-2014, In : Journal of Nuclear Medicine. 55, 8, p. 1330-1335 6 p.

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  • PET Imaging of Disease Progression and Treatment Effects in the Experimental Autoimmune

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DOI

  • Daniele de Paula Faria
  • Maria L. H. Vlaming
  • Sjef C. V. M. Copray
  • Frans Tielen
  • Herma J. A. Anthonijsz
  • Jurgen W. A. Sijbesma
  • Carlos A. Buchpiguel
  • Rudi A. J. O. Dierckx
  • Jose W. A. van der Hoorn
  • Erik F. J. de Vries

The experimental autoimmune encephalomyelitis model is a model of multiple sclerosis that closely mimics the disease characteristics in humans. The main hallmarks of multiple sclerosis are neuroinflammation (microglia activation, monocyte invasion, and T-cell infiltration) and demyelination. PET imaging may be a useful non-invasive technique for monitoring disease progression and drug treatment efficacy in vivo. Methods: Experimental autoimmune encephalomyelitis was induced by myelin-oligodendrocyte glycoprotein immunization in female Dark Agouti rats. Experimental autoimmune encephalomyelitis rats were imaged at baseline and at days 6, 11, 15, and 19 after immunization to monitor monocyte and microglia activation (C-11-PK11195) and demyelination (C-11-MeDAS) during normal disease progression and during treatment with dexamethasone. Results: C-11-PK11195 PET detected activation of microglia and monocytes in the brain stem and spinal cord during disease progression. The uptake of C-11-PK11195 was elevated in dexamethasone-treated animals that had shown mild clinical symptoms that had resolved at the time of imaging. Demyelination was not detected by C-11-MeDAS PET, probably because of the small size of the lesions (average, 0.13 mm). Conclusion: PET imaging of neuroinflammation can be used to monitor disease progression and the consequences of treatment in the experimental autoimmune encephalomyelitis rat model. PET imaging was more sensitive than clinical symptoms for detecting inflammatory changes in the central nervous system.

Original languageEnglish
Pages (from-to)1330-1335
Number of pages6
JournalJournal of Nuclear Medicine
Volume55
Issue number8
Publication statusPublished - Aug-2014

    Keywords

  • multiple sclerosis, PET imaging, neuroinflammation, demyelination, POSITRON-EMISSION-TOMOGRAPHY, PERIPHERAL BENZODIAZEPINE-RECEPTOR, IN-VIVO QUANTIFICATION, MULTIPLE-SCLEROSIS, SPINAL-CORD, TRANSLOCATOR PROTEIN, NERVOUS-SYSTEM, MYELIN, ACTIVATION, EXPRESSION

ID: 14255987