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Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation

Nagotu, S., Saraya, R., Otzen, M., Veenhuis, M. & van der Klei, I. J., May-2008, In : Biochimica et Biophysica Acta-Molecular Cell Research. 1783, 5, p. 760-769 10 p.

Research output: Contribution to journal › Article › Academic › peer-review

APA

Nagotu, S., Saraya, R., Otzen, M., Veenhuis, M., & van der Klei, I. J. (2008). Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation. Biochimica et Biophysica Acta-Molecular Cell Research, 1783(5), 760-769. https://doi.org/10.1016/j.bbamcr.2007.10.018

Author

Nagotu, Shinisha ; Saraya, Ruchi ; Otzen, Marleen ; Veenhuis, Marten ; van der Klei, Ida J. / Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation. In: Biochimica et Biophysica Acta-Molecular Cell Research. 2008 ; Vol. 1783, No. 5. pp. 760-769.

Harvard

Nagotu, S, Saraya, R, Otzen, M, Veenhuis, M & van der Klei, IJ 2008, 'Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation', Biochimica et Biophysica Acta-Molecular Cell Research, vol. 1783, no. 5, pp. 760-769. https://doi.org/10.1016/j.bbamcr.2007.10.018

Standard

Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation. / Nagotu, Shinisha; Saraya, Ruchi; Otzen, Marleen; Veenhuis, Marten; van der Klei, Ida J.

In: Biochimica et Biophysica Acta-Molecular Cell Research, Vol. 1783, No. 5, 05.2008, p. 760-769.

Research output: Contribution to journal › Article › Academic › peer-review

Vancouver

Nagotu S, Saraya R, Otzen M, Veenhuis M, van der Klei IJ. Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation. Biochimica et Biophysica Acta-Molecular Cell Research. 2008 May;1783(5):760-769. https://doi.org/10.1016/j.bbamcr.2007.10.018

BibTeX

@article{8eba78f889184be0b55f77ea48b8fa60,
title = "Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation",
abstract = "We show that the dynamin-like proteins Dnm1p and Vps1p are not required for re-introduction of peroxisomes in Hansenula polymorpha pex3 cells upon complementation with PEX3-GFP. Instead, Dnm1p, but not Vps1p, plays a crucial role in organelle proliferation via fission. In H. polymorpha DNM1 deletion cells (dnm1) a single peroxisome is present that forms long extensions, which protrude into developing buds and divide during cytokinesis. Budding pex11.dnm1 double deletion cells lack these peroxisomal extensions, suggesting that the peroxisomal membrane protein Pex11p is required for their formation. Life cell imaging revealed that fluorescent Dnm1p-GFP spots fluctuate between peroxisomes and mitochondria. On the other hand Pex11p is present over the entire organelle surface, but concentrates during fission at the basis of the organelle extension in dnm1 cells. Our data indicate that peroxisome fission is the major pathway for peroxisome multiplication in H. polymorpha. (C) 2007 Elsevier B.V. All rights reserved.",
keywords = "peroxisome, yeast, dynamin-like protein, Dnm1p, Pex11p, DYNAMIN-RELATED GTPASE, SACCHAROMYCES-CEREVISIAE, MITOCHONDRIAL FISSION, ENDOPLASMIC-RETICULUM, PROTEIN, DIVISION, YEAST, MEMBRANE, PEX3P, BIOGENESIS",
author = "Shinisha Nagotu and Ruchi Saraya and Marleen Otzen and Marten Veenhuis and {van der Klei}, {Ida J.}",
year = "2008",
month = "5",
doi = "10.1016/j.bbamcr.2007.10.018",
language = "English",
volume = "1783",
pages = "760--769",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "ELSEVIER SCIENCE BV",
number = "5",

}

RIS

TY - JOUR

T1 - Peroxisome proliferation in Hansenula polymorpha requires Dnm1p which mediates fission but not de novo formation

AU - Nagotu, Shinisha

AU - Saraya, Ruchi

AU - Otzen, Marleen

AU - Veenhuis, Marten

AU - van der Klei, Ida J.

PY - 2008/5

Y1 - 2008/5

N2 - We show that the dynamin-like proteins Dnm1p and Vps1p are not required for re-introduction of peroxisomes in Hansenula polymorpha pex3 cells upon complementation with PEX3-GFP. Instead, Dnm1p, but not Vps1p, plays a crucial role in organelle proliferation via fission. In H. polymorpha DNM1 deletion cells (dnm1) a single peroxisome is present that forms long extensions, which protrude into developing buds and divide during cytokinesis. Budding pex11.dnm1 double deletion cells lack these peroxisomal extensions, suggesting that the peroxisomal membrane protein Pex11p is required for their formation. Life cell imaging revealed that fluorescent Dnm1p-GFP spots fluctuate between peroxisomes and mitochondria. On the other hand Pex11p is present over the entire organelle surface, but concentrates during fission at the basis of the organelle extension in dnm1 cells. Our data indicate that peroxisome fission is the major pathway for peroxisome multiplication in H. polymorpha. (C) 2007 Elsevier B.V. All rights reserved.

AB - We show that the dynamin-like proteins Dnm1p and Vps1p are not required for re-introduction of peroxisomes in Hansenula polymorpha pex3 cells upon complementation with PEX3-GFP. Instead, Dnm1p, but not Vps1p, plays a crucial role in organelle proliferation via fission. In H. polymorpha DNM1 deletion cells (dnm1) a single peroxisome is present that forms long extensions, which protrude into developing buds and divide during cytokinesis. Budding pex11.dnm1 double deletion cells lack these peroxisomal extensions, suggesting that the peroxisomal membrane protein Pex11p is required for their formation. Life cell imaging revealed that fluorescent Dnm1p-GFP spots fluctuate between peroxisomes and mitochondria. On the other hand Pex11p is present over the entire organelle surface, but concentrates during fission at the basis of the organelle extension in dnm1 cells. Our data indicate that peroxisome fission is the major pathway for peroxisome multiplication in H. polymorpha. (C) 2007 Elsevier B.V. All rights reserved.

KW - peroxisome

KW - yeast

KW - dynamin-like protein

KW - Dnm1p

KW - Pex11p

KW - DYNAMIN-RELATED GTPASE

KW - SACCHAROMYCES-CEREVISIAE

KW - MITOCHONDRIAL FISSION

KW - ENDOPLASMIC-RETICULUM

KW - PROTEIN

KW - DIVISION

KW - YEAST

KW - MEMBRANE

KW - PEX3P

KW - BIOGENESIS

U2 - 10.1016/j.bbamcr.2007.10.018

DO - 10.1016/j.bbamcr.2007.10.018

M3 - Article

VL - 1783

SP - 760

EP - 769

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 5

ER -

ID: 4722629

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