Peroxisome fission in Hansenula polymorpha requires Mdv1 and Fis1, two proteins also involved in mitochondrial fission

Nagotu, S., Krikken, A. M., Otzen, M., Kiel, J. A. K. W., Veenhuis, M. & van der Klei, I. J., Sep-2008, In : Traffic. 9, 9, p. 1471-1484 14 p.

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We show that Mdv1 and Caf4, two components of the mitochondrial fission machinery in Saccharomyces cerevisiae, also function in peroxisome proliferation. Deletion of MDV1, CAF4 or both, however, had only a minor effect on peroxisome numbers at peroxisome-inducing growth conditions, most likely related to the fact that Vps1--and not Dnm1--is the key player in peroxisome fission in this organism. In contrast, in Hansenula polymorpha, which has only a Dnm1-dependent peroxisome fission machinery, deletion of MDV1 led to a drastic reduction of peroxisome numbers. This phenotype was accompanied by a strong defect in mitochondrial fission. The MDV1 paralog CAF4 is absent in H. polymorpha. In wild-type H. polymorpha, cells Dnm1-mCherry and green fluorescent protein (GFP)-Mdv1 colocalize in spots that associate with both peroxisomes and mitochondria. Furthermore, Fis1 is essential to recruit Mdv1 to the peroxisomal and mitochondrial membrane. However, formation of GFP-Mdv1 spots--and related to this normal organelle fission--is strictly dependent on the presence of Dnm1. In dnm1 cells, GFP-Mdv1 is dispersed over the surface of peroxisomes and mitochondria. Also, in H. polymorpha mdv1 or fis1 cells, the number of Dnm1-GFP spots is strongly reduced. These spots still associate to organelles but are functionally inactive.

Original languageEnglish
Pages (from-to)1471-1484
Number of pages14
Issue number9
Publication statusPublished - Sep-2008


  • Base Sequence, Dynamins, Fungal Proteins, Green Fluorescent Proteins, Microscopy, Fluorescence, Mitochondria, Mitochondrial Proteins, Molecular Sequence Data, Mutation, Peroxisomes, Pichia, Plasmids, Protein Transport, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins

ID: 4764302