Publication

Peroxisome biogenesis and dynamics in Hansenula polymorpha

Krygowska, M., 2014, [S.l.]: s.n.. 182 p.

Research output: ThesisThesis fully internal (DIV)

APA

Krygowska, M. (2014). Peroxisome biogenesis and dynamics in Hansenula polymorpha. s.n.

Author

Krygowska, Malgorzata. / Peroxisome biogenesis and dynamics in Hansenula polymorpha. [S.l.] : s.n., 2014. 182 p.

Harvard

Krygowska, M 2014, 'Peroxisome biogenesis and dynamics in Hansenula polymorpha', Doctor of Philosophy, University of Groningen, [S.l.].

Standard

Peroxisome biogenesis and dynamics in Hansenula polymorpha. / Krygowska, Malgorzata.

[S.l.] : s.n., 2014. 182 p.

Research output: ThesisThesis fully internal (DIV)

Vancouver

Krygowska M. Peroxisome biogenesis and dynamics in Hansenula polymorpha. [S.l.]: s.n., 2014. 182 p.


BibTeX

@phdthesis{7e636b1e26044acab939087732ddc427,
title = "Peroxisome biogenesis and dynamics in Hansenula polymorpha",
abstract = "Peroxisomes are important organelles to sustain life, also in man. They are very flexible with respect to their metabolic function and readily adapt their function in relation to metabolic needs. This thesis describes studies on the development and dynamics of peroxisomes in the methylotrophic yeast Hansenula polymorpha.In yeast, peroxisomes multiply by asymmetric fission. We observed that during fission a distinct set of peroxisomal membrane proteins (PMPs) is unevenly distributed over the mother and daughter peroxisomes. As a result the new, small organelles are formed that are fully equipped to rapidly grow and incorporate novel matrix proteins to perform their function in the new cellNext, we studied the process of de novo peroxisome formation. Until now, pex3 cells were assumed to lack peroxisomal structures and used the ER as membrane template for de novo peroxisome creation Unexpected, we observed vesicular structures in pex3 cells that developed into normal peroxisomes upon re-introduction of Pex3. This groundbreaking discovery implies that the current models on peroxisome de novo synthesis and PMP sorting are no longer valid and require adaptation Additionally, we studied the involvement of the H. polymorpha Pex23 protein family (Pex23 and Pex32) in peroxisome biology. We discovered that Pex32 has a major impact on organelle biogenesis, whereas Pex23 most probably stimulates de novo peroxisome formation.Finally, we identified a bona fide homologue of S. cerevisiae Inp2 in H. polymorpha, which plays a role in peroxisome distribution over mother and daughter cells during cell division.",
author = "Malgorzata Krygowska",
year = "2014",
language = "English",
isbn = "978-90-367-6953-2",
publisher = "s.n.",
school = "University of Groningen",

}

RIS

TY - THES

T1 - Peroxisome biogenesis and dynamics in Hansenula polymorpha

AU - Krygowska, Malgorzata

PY - 2014

Y1 - 2014

N2 - Peroxisomes are important organelles to sustain life, also in man. They are very flexible with respect to their metabolic function and readily adapt their function in relation to metabolic needs. This thesis describes studies on the development and dynamics of peroxisomes in the methylotrophic yeast Hansenula polymorpha.In yeast, peroxisomes multiply by asymmetric fission. We observed that during fission a distinct set of peroxisomal membrane proteins (PMPs) is unevenly distributed over the mother and daughter peroxisomes. As a result the new, small organelles are formed that are fully equipped to rapidly grow and incorporate novel matrix proteins to perform their function in the new cellNext, we studied the process of de novo peroxisome formation. Until now, pex3 cells were assumed to lack peroxisomal structures and used the ER as membrane template for de novo peroxisome creation Unexpected, we observed vesicular structures in pex3 cells that developed into normal peroxisomes upon re-introduction of Pex3. This groundbreaking discovery implies that the current models on peroxisome de novo synthesis and PMP sorting are no longer valid and require adaptation Additionally, we studied the involvement of the H. polymorpha Pex23 protein family (Pex23 and Pex32) in peroxisome biology. We discovered that Pex32 has a major impact on organelle biogenesis, whereas Pex23 most probably stimulates de novo peroxisome formation.Finally, we identified a bona fide homologue of S. cerevisiae Inp2 in H. polymorpha, which plays a role in peroxisome distribution over mother and daughter cells during cell division.

AB - Peroxisomes are important organelles to sustain life, also in man. They are very flexible with respect to their metabolic function and readily adapt their function in relation to metabolic needs. This thesis describes studies on the development and dynamics of peroxisomes in the methylotrophic yeast Hansenula polymorpha.In yeast, peroxisomes multiply by asymmetric fission. We observed that during fission a distinct set of peroxisomal membrane proteins (PMPs) is unevenly distributed over the mother and daughter peroxisomes. As a result the new, small organelles are formed that are fully equipped to rapidly grow and incorporate novel matrix proteins to perform their function in the new cellNext, we studied the process of de novo peroxisome formation. Until now, pex3 cells were assumed to lack peroxisomal structures and used the ER as membrane template for de novo peroxisome creation Unexpected, we observed vesicular structures in pex3 cells that developed into normal peroxisomes upon re-introduction of Pex3. This groundbreaking discovery implies that the current models on peroxisome de novo synthesis and PMP sorting are no longer valid and require adaptation Additionally, we studied the involvement of the H. polymorpha Pex23 protein family (Pex23 and Pex32) in peroxisome biology. We discovered that Pex32 has a major impact on organelle biogenesis, whereas Pex23 most probably stimulates de novo peroxisome formation.Finally, we identified a bona fide homologue of S. cerevisiae Inp2 in H. polymorpha, which plays a role in peroxisome distribution over mother and daughter cells during cell division.

M3 - Thesis fully internal (DIV)

SN - 978-90-367-6953-2

PB - s.n.

CY - [S.l.]

ER -

ID: 11131332