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Perivascular scaffolds loaded with adipose tissue-derived stromal cells attenuate development and progression of abdominal aortic aneurysm in rats

Parvizi, M., Petersen, A. H., van Spreuwel-Goossens, C. A. F. M., Kluijtmans, S. G. J. M. & Harmsen, M. C., Sep-2018, In : Journal of Biomedical Materials Research. Part A. 106, 9, p. 2494-2506 13 p.

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  • Perivascular scaffolds loaded with adipose tissue

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DOI

Abdominal aortic aneurysm (AAA) is the pathological dilation and weakening of the abdominal aorta wall. Inflammation, degradation of the extracellular matrix (ECM) and loss of smooth muscle cells and skewing of their function are pivotal in AAA pathology. We developed a recombinant collagen-based patch (RCP) to provide structural integrity and deliver Adipose tissue-Derived Stromal Cells (ASC) for repair. Patches supported adhesion and function as well as proliferation of ASC. ASC-loaded RCPs or bare patches, applied around the aorta after AAA induction in rats, both maintained structural integrity of the aortic wall at time of explant (2w). However, wall thinning, accompanied by loss of elastin fibers and loss of medial SMC, was only attenuated in ASC-loaded RCP-treated AAA rats. Interestingly, this coincided with migration of ASC into the media and a reduced influx of macrophages. We hypothesize that the medially-migrated ASC dampened or skewed the adverse innate immunity and thus suppressed SMC apoptosis, phenotypic skewing and elastin degradation. We conclude that the periadventitial delivery of ASC with RCP suppresses development and progression of AAA, which is has an expected future clinical benefit in combination with an appropriate early screening program of patients at risk for aneurysms. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A:2494-2506, 2018.

Original languageEnglish
Pages (from-to)2494-2506
Number of pages13
JournalJournal of Biomedical Materials Research. Part A
Volume106
Issue number9
Publication statusPublished - Sep-2018

    Keywords

  • aortic aneurysm, ASC, cell therapy, biomaterial, scaffold, SMOOTH-MUSCLE-CELLS, MESENCHYMAL STEM-CELLS, MAST-CELLS, MEDIAL NEOVASCULARIZATION, INFLAMMATION, WALL, GROWTH, REPAIR, MODEL, DOXYCYCLINE

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