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Patterns of divergence across the geographic and genomic landscape of a butterfly hybrid zone associated with a climatic gradient

Ryan, S., Fontaine, M., Scriber, M., Pfrender, M., O'Neil, S. & Hellmann, J. 26-Sep-2017 In : Molecular Ecology. 26, 18, p. 4725-4742 18 p.

Research output: Scientific - peer-reviewArticle

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  • Patterns of divergence across the geographic and genomic landscape of a butterfly hybrid 1 zone associated with a climatic gradient

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    Embargo ends: 19/06/2018

  • Patterns of divergence across the geographic and genomic landscape of a butterfly hybrid zone associated with a climatic gradient

    Final publisher's version, 1 MB, PDF-document

DOI

  • Sean Ryan
  • Michael Fontaine
  • Mark Scriber
  • Michael Pfrender
  • Shawn O'Neil
  • Jessica Hellmann

Hybrid zones are a valuable tool for studying the process of speciation and for identifying the genomic regions undergoing divergence and the ecological (extrinsic) and nonecological (intrinsic) factors involved. Here, we explored the genomic and geographic landscape of divergence in a hybrid zone between Papilio glaucus and Papilio canadensis. Using a genome scan of 28,417 ddRAD SNPs, we identified genomic regions under possible selection and examined their distribution in the context of previously identified candidate genes for ecological adaptations. We showed that differentiation was genomewide, including multiple candidate genes for ecological adaptations, particularly those involved in seasonal adaptation and host plant detoxification. The Z chromosome and four autosomes showed a disproportionate amount of differentiation, suggesting genes on these chromosomes play a potential role in reproductive isolation. Cline analyses of significantly differentiated genomic SNPs, and of species-diagnostic genetic markers, showed a high degree of geographic coincidence (81%) and concordance (80%) and were associated with the geographic distribution of a climate-mediated developmental threshold (length of the growing season). A relatively large proportion (1.3%) of the outliers for divergent selection were not associated with candidate genes for ecological adaptations and may reflect the presence of previously unrecognized intrinsic barriers between these species. These results suggest that exogenous (climate-mediated) and endogenous (unknown) clines may have become coupled and act together to reinforce reproductive isolation. This approach of assessing divergence across both the genomic and geographic landscape can provide insight about the interplay between the genetic architecture of reproductive isolation and endogenous and exogenous selection.

Original languageEnglish
Pages (from-to)4725-4742
Number of pages18
JournalMolecular Ecology
Volume26
Issue number18
StatePublished - 26-Sep-2017

    Keywords

  • climate, cline, genomic, hybridization, intrinsic incompatibilities, RAD, TIGER SWALLOWTAIL BUTTERFLIES, PAPILIO-GLAUCUS-CANADENSIS, MULTILOCUS GENOTYPE DATA, DNA-SEQUENCING DATA, FIRE-BELLIED TOADS, POPULATION-STRUCTURE, DROSOPHILA-MELANOGASTER, GENE FLOW, HOST-USE, CYTOCHROME-P450 MONOOXYGENASES

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