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PAS7 ENCODES A NOVEL YEAST MEMBER OF THE WD-40 PROTEIN FAMILY ESSENTIAL FOR IMPORT OF 3-OXOACYL-COA THIOLASE, A PTS2-CONTAINING PROTEIN, INTO PEROXISOMES

Marzioch, M., Erdmann, R., Veenhuis, M. & Kunau, W-H., 17-Oct-1994, In : EMBO Journal. 13, 20, p. 4908-4918 11 p.

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Documents

  • Martina Marzioch
  • Ralf Erdmann
  • Marten Veenhuis
  • Wolf-H. Kunau
To identify components of the peroxisomal import pathway in yeast, we have isolated pas mutants affected in peroxisome biogenesis. Two mutants assigned to complementation group 7 define a new gene, PAS7, whose product is necessary for import of thiolase, a PTS2-containing protein, but not for that of SKL (PTS1)-containing proteins, into peroxisomes. We have cloned PAS7 by complementation of the oleic acid non-utilizing phenotype of the pas7-1 strain. The DNA sequence predicts a 42.3 kDa polypeptide of 375 amino acids encoding a novel member of the P-transducin related (WD-40) protein family. A Myc epitope-tagged Pas7p, expressed under the control of the CUP1 promotor, was functionally active. Subcellular localization studies revealed that in the presence of thiolase this epitope-tagged Pas7p in part associates with peroxisomes. However, in a thiolase-deficient mutant, Pas7p was entirely found in the cytoplasm. We suggest that Pas7p mediates the binding of thiolase to these organelles.
Original languageEnglish
Pages (from-to)4908-4918
Number of pages11
JournalEMBO Journal
Volume13
Issue number20
Publication statusPublished - 17-Oct-1994

    Keywords

  • 3-OXOACYL-COA THIOLASE, PEROXISOMAL TARGETING SIGNAL, PEROXISOME, PROTEIN SORTING, SACCHAROMYCES CEREVISIAE, POSITIVE SELECTION PROCEDURE, COLI SHUTTLE VECTORS, SACCHAROMYCES-CEREVISIAE, 3-KETOACYL-COA THIOLASE, TARGETING SIGNAL, HANSENULA-POLYMORPHA, TERMINAL PRESEQUENCE, CANDIDA-TROPICALIS, FIREFLY LUCIFERASE, ZELLWEGER SYNDROME

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