Publication

Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

Sokol, E., Nijenhuis, M., Sjollema, K. A., Jonkman, M. F., Pas, H. H. & Giepmans, B. N. G., 2017, Inflammation: Methods and Protocols. Clausen, B. E. & Laman, J. D. (eds.). 1 ed. Springer, Vol. 1559. p. 107-118 12 p.

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

APA

Sokol, E., Nijenhuis, M., Sjollema, K. A., Jonkman, M. F., Pas, H. H., & Giepmans, B. N. G. (2017). Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins. In B. E. Clausen, & J. D. Laman (Eds.), Inflammation: Methods and Protocols (1 ed., Vol. 1559, pp. 107-118). Springer. https://doi.org/10.1007/978-1-4939-6786-5_9

Author

Sokol, Ena ; Nijenhuis, Miranda ; Sjollema, Klaas A ; Jonkman, Marcel F ; Pas, Hendri H ; Giepmans, Ben N G. / Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins. Inflammation: Methods and Protocols. editor / Björn E. Clausen ; Jon D. Laman. Vol. 1559 1. ed. Springer, 2017. pp. 107-118

Harvard

Sokol, E, Nijenhuis, M, Sjollema, KA, Jonkman, MF, Pas, HH & Giepmans, BNG 2017, Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins. in BE Clausen & JD Laman (eds), Inflammation: Methods and Protocols. 1 edn, vol. 1559, Springer, pp. 107-118. https://doi.org/10.1007/978-1-4939-6786-5_9

Standard

Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins. / Sokol, Ena; Nijenhuis, Miranda; Sjollema, Klaas A; Jonkman, Marcel F; Pas, Hendri H; Giepmans, Ben N G.

Inflammation: Methods and Protocols. ed. / Björn E. Clausen; Jon D. Laman. Vol. 1559 1. ed. Springer, 2017. p. 107-118.

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

Vancouver

Sokol E, Nijenhuis M, Sjollema KA, Jonkman MF, Pas HH, Giepmans BNG. Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins. In Clausen BE, Laman JD, editors, Inflammation: Methods and Protocols. 1 ed. Vol. 1559. Springer. 2017. p. 107-118 https://doi.org/10.1007/978-1-4939-6786-5_9


BibTeX

@inbook{333a4e3cd5d24cd4acd9d91444738c6f,
title = "Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins",
abstract = "Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.",
author = "Ena Sokol and Miranda Nijenhuis and Sjollema, {Klaas A} and Jonkman, {Marcel F} and Pas, {Hendri H} and Giepmans, {Ben N G}",
year = "2017",
doi = "10.1007/978-1-4939-6786-5_9",
language = "English",
isbn = "978-1-4939-6784-1",
volume = "1559",
pages = "107--118",
editor = "Clausen, {Bj{\"o}rn E.} and Laman, {Jon D.}",
booktitle = "Inflammation",
publisher = "Springer",
edition = "1",

}

RIS

TY - CHAP

T1 - Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins

AU - Sokol, Ena

AU - Nijenhuis, Miranda

AU - Sjollema, Klaas A

AU - Jonkman, Marcel F

AU - Pas, Hendri H

AU - Giepmans, Ben N G

PY - 2017

Y1 - 2017

N2 - Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

AB - Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.

U2 - 10.1007/978-1-4939-6786-5_9

DO - 10.1007/978-1-4939-6786-5_9

M3 - Chapter

C2 - 28063041

SN - 978-1-4939-6784-1

VL - 1559

SP - 107

EP - 118

BT - Inflammation

A2 - Clausen, Björn E.

A2 - Laman, Jon D.

PB - Springer

ER -

ID: 41915337