Publication

Parental DNA methylation states are associated with heterosis in epigenetic hybrids

Lauss, K., Wardenaar, R., Oka, R., van Hulten, M. H. A., Guryev, V., Keurentjes, J. J. B., Stam, M. & Johannes, F., Feb-2018, In : Plant Physiology. 176, 4, p. 1627-1645 19 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Lauss, K., Wardenaar, R., Oka, R., van Hulten, M. H. A., Guryev, V., Keurentjes, J. J. B., ... Johannes, F. (2018). Parental DNA methylation states are associated with heterosis in epigenetic hybrids. Plant Physiology, 176(4), 1627-1645. https://doi.org/10.1104/pp.17.01054

Author

Lauss, Kathrin ; Wardenaar, René ; Oka, Rurika ; van Hulten, Marieke H A ; Guryev, Victor ; Keurentjes, Joost J B ; Stam, Maike ; Johannes, Frank. / Parental DNA methylation states are associated with heterosis in epigenetic hybrids. In: Plant Physiology. 2018 ; Vol. 176, No. 4. pp. 1627-1645.

Harvard

Lauss, K, Wardenaar, R, Oka, R, van Hulten, MHA, Guryev, V, Keurentjes, JJB, Stam, M & Johannes, F 2018, 'Parental DNA methylation states are associated with heterosis in epigenetic hybrids', Plant Physiology, vol. 176, no. 4, pp. 1627-1645. https://doi.org/10.1104/pp.17.01054

Standard

Parental DNA methylation states are associated with heterosis in epigenetic hybrids. / Lauss, Kathrin; Wardenaar, René; Oka, Rurika; van Hulten, Marieke H A; Guryev, Victor; Keurentjes, Joost J B; Stam, Maike; Johannes, Frank.

In: Plant Physiology, Vol. 176, No. 4, 02.2018, p. 1627-1645.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Lauss K, Wardenaar R, Oka R, van Hulten MHA, Guryev V, Keurentjes JJB et al. Parental DNA methylation states are associated with heterosis in epigenetic hybrids. Plant Physiology. 2018 Feb;176(4):1627-1645. https://doi.org/10.1104/pp.17.01054


BibTeX

@article{e925562416bc43179946774feb376203,
title = "Parental DNA methylation states are associated with heterosis in epigenetic hybrids",
abstract = "Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. Recent studies have implicated changes in DNA methylation and small RNAs in hybrid performance, however, it remains unclear whether epigenetic changes are a cause or consequence of heterosis. Here, we analyze a large panel of over 500 A. thaliana epigenetic hybrid plants (epiHybrids), which we derived from near-isogenic but epigenetically divergent parents. This proof-of-principle experimental system allowed us to quantify the contribution of parental methylation differences to heterosis. We measured traits such as leaf area (LA), growth rate (GR), flowering time (FT), main stem branching (MSB), rosette branching (RB) and final plant height (HT) and observed several strong positive and negative heterotic phenotypes among the epiHybrids. Using an epigenetic quantitative trait locus mapping approach, we were able to identify specific differentially methylated regions (DMRs) in the parental genomes that are associated with hybrid performance. Sequencing of methylomes, transcriptomes and genomes of selected parent-epiHybrid combinations further showed that these parental DMRs most likely mediate remodeling of methylation and transcriptional states at specific loci in the hybrids. Taken together, our data suggest that locus-specific epigenetic divergence between the parental lines can directly or indirectly trigger heterosis in Arabidopsis hybrids independent of genetic changes. These results add to a growing body of evidence that points to epigenetic factors as one of the key determinants of hybrid performance.",
keywords = "DNA methylation, heterosis, epigentics, small RNAs, Arabidopsis thaliana, PLANTS, DEFENSE, TRANS CHROMOSOMAL METHYLATION, ALTERED CIRCADIAN-RHYTHMS, ARABIDOPSIS F1 HYBRIDS, READ ALIGNMENT, GROWTH, VIGOR, THALIANA, PATTERNS",
author = "Kathrin Lauss and Ren{\'e} Wardenaar and Rurika Oka and {van Hulten}, {Marieke H A} and Victor Guryev and Keurentjes, {Joost J B} and Maike Stam and Frank Johannes",
note = "{copyright, serif} 2017 American Society of Plant Biologists. All rights reserved.",
year = "2018",
month = "2",
doi = "10.1104/pp.17.01054",
language = "English",
volume = "176",
pages = "1627--1645",
journal = "Plant Physiology",
issn = "1532-2548",
publisher = "AMER SOC PLANT BIOLOGISTS",
number = "4",

}

RIS

TY - JOUR

T1 - Parental DNA methylation states are associated with heterosis in epigenetic hybrids

AU - Lauss, Kathrin

AU - Wardenaar, René

AU - Oka, Rurika

AU - van Hulten, Marieke H A

AU - Guryev, Victor

AU - Keurentjes, Joost J B

AU - Stam, Maike

AU - Johannes, Frank

N1 - {copyright, serif} 2017 American Society of Plant Biologists. All rights reserved.

PY - 2018/2

Y1 - 2018/2

N2 - Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. Recent studies have implicated changes in DNA methylation and small RNAs in hybrid performance, however, it remains unclear whether epigenetic changes are a cause or consequence of heterosis. Here, we analyze a large panel of over 500 A. thaliana epigenetic hybrid plants (epiHybrids), which we derived from near-isogenic but epigenetically divergent parents. This proof-of-principle experimental system allowed us to quantify the contribution of parental methylation differences to heterosis. We measured traits such as leaf area (LA), growth rate (GR), flowering time (FT), main stem branching (MSB), rosette branching (RB) and final plant height (HT) and observed several strong positive and negative heterotic phenotypes among the epiHybrids. Using an epigenetic quantitative trait locus mapping approach, we were able to identify specific differentially methylated regions (DMRs) in the parental genomes that are associated with hybrid performance. Sequencing of methylomes, transcriptomes and genomes of selected parent-epiHybrid combinations further showed that these parental DMRs most likely mediate remodeling of methylation and transcriptional states at specific loci in the hybrids. Taken together, our data suggest that locus-specific epigenetic divergence between the parental lines can directly or indirectly trigger heterosis in Arabidopsis hybrids independent of genetic changes. These results add to a growing body of evidence that points to epigenetic factors as one of the key determinants of hybrid performance.

AB - Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. Recent studies have implicated changes in DNA methylation and small RNAs in hybrid performance, however, it remains unclear whether epigenetic changes are a cause or consequence of heterosis. Here, we analyze a large panel of over 500 A. thaliana epigenetic hybrid plants (epiHybrids), which we derived from near-isogenic but epigenetically divergent parents. This proof-of-principle experimental system allowed us to quantify the contribution of parental methylation differences to heterosis. We measured traits such as leaf area (LA), growth rate (GR), flowering time (FT), main stem branching (MSB), rosette branching (RB) and final plant height (HT) and observed several strong positive and negative heterotic phenotypes among the epiHybrids. Using an epigenetic quantitative trait locus mapping approach, we were able to identify specific differentially methylated regions (DMRs) in the parental genomes that are associated with hybrid performance. Sequencing of methylomes, transcriptomes and genomes of selected parent-epiHybrid combinations further showed that these parental DMRs most likely mediate remodeling of methylation and transcriptional states at specific loci in the hybrids. Taken together, our data suggest that locus-specific epigenetic divergence between the parental lines can directly or indirectly trigger heterosis in Arabidopsis hybrids independent of genetic changes. These results add to a growing body of evidence that points to epigenetic factors as one of the key determinants of hybrid performance.

KW - DNA methylation

KW - heterosis

KW - epigentics

KW - small RNAs

KW - Arabidopsis thaliana

KW - PLANTS

KW - DEFENSE

KW - TRANS CHROMOSOMAL METHYLATION

KW - ALTERED CIRCADIAN-RHYTHMS

KW - ARABIDOPSIS F1 HYBRIDS

KW - READ ALIGNMENT

KW - GROWTH

KW - VIGOR

KW - THALIANA

KW - PATTERNS

U2 - 10.1104/pp.17.01054

DO - 10.1104/pp.17.01054

M3 - Article

VL - 176

SP - 1627

EP - 1645

JO - Plant Physiology

JF - Plant Physiology

SN - 1532-2548

IS - 4

ER -

ID: 51310015