Publication

Parental DNA methylation states are associated with heterosis in epigenetic hybrids

Lauss, K., Wardenaar, R., Oka, R., van Hulten, M. H. A., Guryev, V., Keurentjes, J. J. B., Stam, M. & Johannes, F., Feb-2018, In : Plant Physiology. 176, 4, p. 1627-1645 19 p.

Research output: Contribution to journalArticleAcademicpeer-review

  • Kathrin Lauss
  • René Wardenaar
  • Rurika Oka
  • Marieke H A van Hulten
  • Victor Guryev
  • Joost J B Keurentjes
  • Maike Stam
  • Frank Johannes

Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. Recent studies have implicated changes in DNA methylation and small RNAs in hybrid performance, however, it remains unclear whether epigenetic changes are a cause or consequence of heterosis. Here, we analyze a large panel of over 500 A. thaliana epigenetic hybrid plants (epiHybrids), which we derived from near-isogenic but epigenetically divergent parents. This proof-of-principle experimental system allowed us to quantify the contribution of parental methylation differences to heterosis. We measured traits such as leaf area (LA), growth rate (GR), flowering time (FT), main stem branching (MSB), rosette branching (RB) and final plant height (HT) and observed several strong positive and negative heterotic phenotypes among the epiHybrids. Using an epigenetic quantitative trait locus mapping approach, we were able to identify specific differentially methylated regions (DMRs) in the parental genomes that are associated with hybrid performance. Sequencing of methylomes, transcriptomes and genomes of selected parent-epiHybrid combinations further showed that these parental DMRs most likely mediate remodeling of methylation and transcriptional states at specific loci in the hybrids. Taken together, our data suggest that locus-specific epigenetic divergence between the parental lines can directly or indirectly trigger heterosis in Arabidopsis hybrids independent of genetic changes. These results add to a growing body of evidence that points to epigenetic factors as one of the key determinants of hybrid performance.

Original languageEnglish
Pages (from-to)1627-1645
Number of pages19
JournalPlant Physiology
Volume176
Issue number4
Early online date1-Dec-2017
Publication statusPublished - Feb-2018

    Keywords

  • DNA methylation, heterosis, epigentics, small RNAs, Arabidopsis thaliana, PLANTS, DEFENSE, TRANS CHROMOSOMAL METHYLATION, ALTERED CIRCADIAN-RHYTHMS, ARABIDOPSIS F1 HYBRIDS, READ ALIGNMENT, GROWTH, VIGOR, THALIANA, PATTERNS

Download statistics

No data available

ID: 51310015