Organic field-effect transistor-based biosensors functionalized with protein receptors

Maddalena, F., Kuiper, M. J., Poolman, B., Brouwer, F., Hummelen, J. C., de Leeuw, D. M., de Boer, B. & Blom, P. W. M., 15-Dec-2010, In : Journal of Applied Physics. 108, 12, p. 124501-1-124501-4 4 p., 124501.

Research output: Contribution to journalArticleAcademicpeer-review

An organic field-effect transistor with integrated proteins (Bio-FET) for sensing of sulfate ions is presented. A sulfate receptor was engineered to contain a thiol group for surface-anchoring without affecting its binding activity. The modified receptor was covalently coupled to a maleimide-functionalized polystyrene layer, and integrated as gate dielectric in a dual-gate transducer. The binding of sulfate ions in dry conditions was detected by a shift in the threshold voltage. Combined with surface density measurements by atomic force microscopy , an effective charge of −1.7q per protein was found, as expected from the Bio-FET operation model.
Original languageEnglish
Article number124501
Pages (from-to)124501-1-124501-4
Number of pages4
JournalJournal of Applied Physics
Issue number12
Publication statusPublished - 15-Dec-2010



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