Publication

One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples

Gil, A., Zhang, W., Wolters, J. C., Permentier, H., Boer, T., Horvatovich, P., Heiner-Fokkema, M. R., Reijngoud, D-J. & Bischoff, R., Sep-2018, In : Analytical and Bioanalytical Chemistry. 410, 23, p. 5859-5870 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Gil, A., Zhang, W., Wolters, J. C., Permentier, H., Boer, T., Horvatovich, P., ... Bischoff, R. (2018). One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples. Analytical and Bioanalytical Chemistry, 410(23), 5859-5870. https://doi.org/10.1007/s00216-018-1200-x

Author

Gil, Andres ; Zhang, Wenxuan ; Wolters, Justina C ; Permentier, Hjalmar ; Boer, Theo ; Horvatovich, Peter ; Heiner-Fokkema, M Rebecca ; Reijngoud, Dirk-Jan ; Bischoff, Rainer. / One- vs two-phase extraction : re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples. In: Analytical and Bioanalytical Chemistry. 2018 ; Vol. 410, No. 23. pp. 5859-5870.

Harvard

Gil, A, Zhang, W, Wolters, JC, Permentier, H, Boer, T, Horvatovich, P, Heiner-Fokkema, MR, Reijngoud, D-J & Bischoff, R 2018, 'One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples' Analytical and Bioanalytical Chemistry, vol. 410, no. 23, pp. 5859-5870. https://doi.org/10.1007/s00216-018-1200-x

Standard

One- vs two-phase extraction : re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples. / Gil, Andres; Zhang, Wenxuan; Wolters, Justina C; Permentier, Hjalmar; Boer, Theo; Horvatovich, Peter; Heiner-Fokkema, M Rebecca; Reijngoud, Dirk-Jan; Bischoff, Rainer.

In: Analytical and Bioanalytical Chemistry, Vol. 410, No. 23, 09.2018, p. 5859-5870.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Gil A, Zhang W, Wolters JC, Permentier H, Boer T, Horvatovich P et al. One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples. Analytical and Bioanalytical Chemistry. 2018 Sep;410(23):5859-5870. https://doi.org/10.1007/s00216-018-1200-x


BibTeX

@article{3b2304fe0b5045f9885baf5b53d1f11e,
title = "One- vs two-phase extraction: re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples",
abstract = "Lipidomics is a rapidly developing field in modern biomedical research. While LC-MS systems are able to detect most of the known lipid classes in a biological matrix, there is no single technique able to extract all of them simultaneously. In comparison with two-phase extractions, one-phase extraction systems are of particular interest, since they decrease the complexity of the experimental procedure. By using an untargeted lipidomics approach, we explored the differences/similarities between the most commonly used two-phase extraction systems (Folch, Bligh and Dyer, and MTBE) and one of the more recently introduced one-phase extraction systems for lipid analysis based on the MMC solvent mixture (MeOH/MTBE/CHCl3). The four extraction methods were evaluated and thoroughly compared against a pooled extract that qualitatively and quantitatively represents the average of the combined extractions. Our results show that the lipid profile obtained with the MMC system displayed the highest similarity to the pooled extract, indicating that it was most representative of the lipidome in the original sample. Furthermore, it showed better extraction efficiencies for moderate and highly apolar lipid species in comparison with the Folch, Bligh and Dyer, and MTBE extraction systems. Finally, the technical simplicity of the MMC procedure makes this solvent system highly suitable for automated, untargeted lipidomics analysis.",
keywords = "Lipids, Untargeted lipidomics, Extraction, LC-MS, Multivariate analysis, CHROMATOGRAPHY-MASS-SPECTROMETRY, BLOOD-PLASMA, HUMAN URINE, BUME METHOD, MS, PURIFICATION, SYSTEMS, LIPIDS",
author = "Andres Gil and Wenxuan Zhang and Wolters, {Justina C} and Hjalmar Permentier and Theo Boer and Peter Horvatovich and Heiner-Fokkema, {M Rebecca} and Dirk-Jan Reijngoud and Rainer Bischoff",
year = "2018",
month = "9",
doi = "10.1007/s00216-018-1200-x",
language = "English",
volume = "410",
pages = "5859--5870",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "SPRINGER HEIDELBERG",
number = "23",

}

RIS

TY - JOUR

T1 - One- vs two-phase extraction

T2 - re-evaluation of sample preparation procedures for untargeted lipidomics in plasma samples

AU - Gil, Andres

AU - Zhang, Wenxuan

AU - Wolters, Justina C

AU - Permentier, Hjalmar

AU - Boer, Theo

AU - Horvatovich, Peter

AU - Heiner-Fokkema, M Rebecca

AU - Reijngoud, Dirk-Jan

AU - Bischoff, Rainer

PY - 2018/9

Y1 - 2018/9

N2 - Lipidomics is a rapidly developing field in modern biomedical research. While LC-MS systems are able to detect most of the known lipid classes in a biological matrix, there is no single technique able to extract all of them simultaneously. In comparison with two-phase extractions, one-phase extraction systems are of particular interest, since they decrease the complexity of the experimental procedure. By using an untargeted lipidomics approach, we explored the differences/similarities between the most commonly used two-phase extraction systems (Folch, Bligh and Dyer, and MTBE) and one of the more recently introduced one-phase extraction systems for lipid analysis based on the MMC solvent mixture (MeOH/MTBE/CHCl3). The four extraction methods were evaluated and thoroughly compared against a pooled extract that qualitatively and quantitatively represents the average of the combined extractions. Our results show that the lipid profile obtained with the MMC system displayed the highest similarity to the pooled extract, indicating that it was most representative of the lipidome in the original sample. Furthermore, it showed better extraction efficiencies for moderate and highly apolar lipid species in comparison with the Folch, Bligh and Dyer, and MTBE extraction systems. Finally, the technical simplicity of the MMC procedure makes this solvent system highly suitable for automated, untargeted lipidomics analysis.

AB - Lipidomics is a rapidly developing field in modern biomedical research. While LC-MS systems are able to detect most of the known lipid classes in a biological matrix, there is no single technique able to extract all of them simultaneously. In comparison with two-phase extractions, one-phase extraction systems are of particular interest, since they decrease the complexity of the experimental procedure. By using an untargeted lipidomics approach, we explored the differences/similarities between the most commonly used two-phase extraction systems (Folch, Bligh and Dyer, and MTBE) and one of the more recently introduced one-phase extraction systems for lipid analysis based on the MMC solvent mixture (MeOH/MTBE/CHCl3). The four extraction methods were evaluated and thoroughly compared against a pooled extract that qualitatively and quantitatively represents the average of the combined extractions. Our results show that the lipid profile obtained with the MMC system displayed the highest similarity to the pooled extract, indicating that it was most representative of the lipidome in the original sample. Furthermore, it showed better extraction efficiencies for moderate and highly apolar lipid species in comparison with the Folch, Bligh and Dyer, and MTBE extraction systems. Finally, the technical simplicity of the MMC procedure makes this solvent system highly suitable for automated, untargeted lipidomics analysis.

KW - Lipids

KW - Untargeted lipidomics

KW - Extraction

KW - LC-MS

KW - Multivariate analysis

KW - CHROMATOGRAPHY-MASS-SPECTROMETRY

KW - BLOOD-PLASMA

KW - HUMAN URINE

KW - BUME METHOD

KW - MS

KW - PURIFICATION

KW - SYSTEMS

KW - LIPIDS

U2 - 10.1007/s00216-018-1200-x

DO - 10.1007/s00216-018-1200-x

M3 - Article

VL - 410

SP - 5859

EP - 5870

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 23

ER -

ID: 62518109