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Oncogenic β-catenin and PIK3CA instruct network states and cancer phenotypes in intestinal organoids

Riemer, P., Rydenfelt, M., Marks, M., van Eunen, K., Thedieck, K., Herrmann, B. G., Blüthgen, N., Sers, C. & Morkel, M., Jun-2017, In : The Journal of Cell Biology. 216, 6, p. 1567-1577 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

Colorectal cancer is driven by cooperating oncogenic mutations. In this study, we use organotypic cultures derived from transgenic mice inducibly expressing oncogenic beta-catenin and/or PIK3CAH1047R to follow sequential changes in cancer-related signaling networks, intestinal cell metabolism, and physiology in a three-dimensional environment mimicking tissue architecture. Activation of beta-catenin alone results in the formation of highly clonogenic cells that are nonmotile and prone to undergo apoptosis. In contrast, coexpression of stabilized beta-catenin and PIK3CAH1047R gives rise to intestinal cells that are apoptosis-resistant, proliferative, stem cell-like, and motile. Systematic inhibitor treatments of organoids followed by quantitative phenotyping and phosphoprotein analyses uncover key changes in the signaling network topology of intestinal cells after induction of stabilized beta-catenin and PIK3CAH1047R. We find that survival and motility of organoid cells are associated with 4EBP1 and AKT phosphorylation, respectively. Our work defines phenotypes, signaling network states, and vulnerabilities of transgenic intestinal organoids as a novel approach to understanding oncogene activities and guiding the development of targeted therapies.

Original languageEnglish
Pages (from-to)1567-1577
Number of pages11
JournalThe Journal of Cell Biology
Volume216
Issue number6
Publication statusPublished - Jun-2017

    Keywords

  • GLYCOGEN-SYNTHASE KINASE-3, COLORECTAL-CANCER, STEM-CELLS, IN-VITRO, AKT, RAF, PHOSPHORYLATION, INHIBITOR, PATHWAY, ERK

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