On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent

Cordes, T., Vogelsang, J. & Tinnefeld, P., 2009, In : Journal of the American Chemical Society. 131, 14, p. 5018-5019 2 p.

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Recent advances in photobleaching and blinking prevention have aided the advancement of single-molecule and super-resolution fluorescence microscopy. However, a common mechanism of the action of antifading agents such as Trolox is still missing. In this communication we present evidence that Trolox acts in accordance with a mechanism that involves triplet quenching through electron transfer and subsequent recovery of the resulting radical ion by the complementary redox reaction. The required oxidant for this unifying mechanism based on a reducing and oxidizing system (ROXS) is formed via (photo-)reaction with molecular oxygen. We present evidence that this oxidized form is a quinone derivative of Trolox with strong oxidizing properties. These findings shed light on many contradicting results regarding the action of antifading agents and might lead to a common mechanistic understanding of photobleaching and its prevention. Finally, a recipe on the proper use of Trolox as an antifading agent is provided.
Original languageEnglish
Pages (from-to)5018-5019
Number of pages2
JournalJournal of the American Chemical Society
Issue number14
Publication statusPublished - 2009

ID: 14535111