Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans

Mata-Cabana, A., Sin, O., Seinstra, R. I. & Nollen, E. A. A., 20-Oct-2018, In : JMIR research protocols. 8, 20, 11 p., 3053.

Research output: Contribution to journalArticleAcademicpeer-review

C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.

Original languageEnglish
Article number3053
Number of pages11
JournalJMIR research protocols
Issue number20
Publication statusPublished - 20-Oct-2018

ID: 71327881