Publication

Novel modifications of nonribosomal peptides from Brevibacillus laterosporus MG64 and investigation of their mode of action

Li, Z., de Vries, R. H., Chakraborty, P., Song, C., Zhao, X., Scheffers, D-J., Roelfes, G. & Kuipers, O. P., Dec-2020, In : Applied and environmental microbiology. 86, 24, p. 1-14 14 p., e01981-20.

Research output: Contribution to journalArticleAcademicpeer-review

Copy link to clipboard

Documents

  • Novel Modifications of Nonribosomal Peptides fromBrevibacillus laterosporusMG64 and Investigation of TheirMode of Action

    Final publisher's version, 1.34 MB, PDF document

    Embargo ends: 26/03/2021

    Request copy

DOI

Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications, including biocontrol and pharmacy. Brevibacillus spp. are a rich source of NRPs yet have received little attention. In this study, we characterize four novel bogorol variants (bogorols I to L, cationic linear lipopeptides) and four succilins (succilins I to L, containing a succinyl group that is attached to the Orn3/Lys3 in bogorols I to L) from the biocontrol strain Brevibacillus laterosporus MG64. Further investigation revealed that the bogorol family of peptides employs an adenylation pathway for lipoinitiation, different from the usual pattern, which is based on an external ligase and coenzyme A. Moreover, the formation of valinol was proven to be mediated by a terminal reductase domain and a reductase encoded by the bogI gene. Furthermore, succinylation, which is a novel type of modification in the family of bogorols, was discovered. Its occurrence requires a high concentration of the substrate (bogorols), but its responsible enzyme remains unknown. Bogorols display potent activity against both Gram-positive and Gram-negative bacteria. Investigation of their mode of action reveals that bogorols form pores in the cell membrane of both Gram-positive and Gram-negative bacteria. The combination of bogorols and relacidines, another class of NRPs produced by B. laterosporus MG64, displays a synergistic effect on different pathogens, suggesting the great potential of both peptides as well as their producer B. laterosporus MG64 for broad applications. Our study provides a further understanding of the bogorol family of peptides as well as their applications.

Original languageEnglish
Article numbere01981-20
Pages (from-to)1-14
Number of pages14
JournalApplied and environmental microbiology
Volume86
Issue number24
Early online date25-Sep-2020
Publication statusPublished - Dec-2020

    Keywords

  • Brevibacillus laterosporus, biosynthesis, bogorol variants, lipoinitiation, mode of action, reduction, succilins, succinylation, synergy

ID: 134855789