Novel genetic tools for Hansenula polymorpha

Ruchi Saraya; Arjen M Krikken; Jan A K W Kiel; Richard J S Baerends; Marten Veenhuis; Ida J van der Klei

doi:10.1111/j.1567-1364.2011.00772.x

Publication

Novel genetic tools for Hansenula polymorpha

Saraya, R., Krikken, A. M., Kiel, J. A. K. W., Baerends, R. J. S., Veenhuis, M. & van der Klei, I. J., May-2012, In : Fems Yeast Research. 12, 3, p. 271-278 8 p.

Research output: Contribution to journal › Review article › Academic › peer-review

APA

Saraya, R., Krikken, A. M., Kiel, J. A. K. W., Baerends, R. J. S., Veenhuis, M., & van der Klei, I. J. (2012). Novel genetic tools for Hansenula polymorpha. Fems Yeast Research, 12(3), 271-278. https://doi.org/10.1111/j.1567-1364.2011.00772.x

Author

Saraya, Ruchi ; Krikken, Arjen M ; Kiel, Jan A K W ; Baerends, Richard J S ; Veenhuis, Marten ; van der Klei, Ida J. / Novel genetic tools for Hansenula polymorpha. In: Fems Yeast Research. 2012 ; Vol. 12, No. 3. pp. 271-278.

Harvard

Saraya, R, Krikken, AM, Kiel, JAKW, Baerends, RJS, Veenhuis, M & van der Klei, IJ 2012, 'Novel genetic tools for Hansenula polymorpha', Fems Yeast Research, vol. 12, no. 3, pp. 271-278. https://doi.org/10.1111/j.1567-1364.2011.00772.x

Standard

Novel genetic tools for Hansenula polymorpha. / Saraya, Ruchi; Krikken, Arjen M; Kiel, Jan A K W; Baerends, Richard J S; Veenhuis, Marten; van der Klei, Ida J.

In: Fems Yeast Research, Vol. 12, No. 3, 05.2012, p. 271-278.

Research output: Contribution to journal › Review article › Academic › peer-review

Vancouver

Saraya R, Krikken AM, Kiel JAKW, Baerends RJS, Veenhuis M, van der Klei IJ. Novel genetic tools for Hansenula polymorpha. Fems Yeast Research. 2012 May;12(3):271-278. https://doi.org/10.1111/j.1567-1364.2011.00772.x

BibTeX

@article{8d76e6cec49044c5898bc08e538b7b9c,

title = "Novel genetic tools for Hansenula polymorpha",

abstract = "Hansenula polymorpha is an important yeast in industrial biotechnology. In addition, it is extensively used in fundamental research devoted to unravel the principles of peroxisome biology and nitrate assimilation. Here we present an overview of key components of the genetic toolbox for H. polymorpha. In addition, we present new selection markers that we recently implemented in H. polymorpha. We describe novel strategies for the efficient creation of targeted gene deletions and integrations in H. polymorpha. For this, we generated a similar to 80 mutant, deficient in non-homologous end joining, resulting in strongly enhanced efficiency of gene targeting relative to the parental strain. Finally, we show the implementation of Gateway technology and a single-step PCR strategy to create deletions in H. polymorpha.",

keywords = "dominant markers, Gateway technology, genetic tools, methylotrophic yeast, non-homologous end-joining, YEAST EXPRESSION PLATFORMS, MATRIX PROTEIN IMPORT, SACCHAROMYCES-CEREVISIAE, PEROXISOMAL MEMBRANE, METHYLOTROPHIC YEAST, ARXULA-ADENINIVORANS, PICHIA-PASTORIS, ALCOHOL OXIDASE, METHANOL, FISSION",

author = "Ruchi Saraya and Krikken, {Arjen M} and Kiel, {Jan A K W} and Baerends, {Richard J S} and Marten Veenhuis and {van der Klei}, {Ida J}",

year = "2012",

month = may,

doi = "10.1111/j.1567-1364.2011.00772.x",

language = "English",

volume = "12",

pages = "271--278",

journal = "Fems Yeast Research",

issn = "1567-1356",

number = "3",

}

RIS

TY - JOUR

T1 - Novel genetic tools for Hansenula polymorpha

AU - Saraya, Ruchi

AU - Krikken, Arjen M

AU - Kiel, Jan A K W

AU - Baerends, Richard J S

AU - Veenhuis, Marten

AU - van der Klei, Ida J

PY - 2012/5

Y1 - 2012/5

N2 - Hansenula polymorpha is an important yeast in industrial biotechnology. In addition, it is extensively used in fundamental research devoted to unravel the principles of peroxisome biology and nitrate assimilation. Here we present an overview of key components of the genetic toolbox for H. polymorpha. In addition, we present new selection markers that we recently implemented in H. polymorpha. We describe novel strategies for the efficient creation of targeted gene deletions and integrations in H. polymorpha. For this, we generated a similar to 80 mutant, deficient in non-homologous end joining, resulting in strongly enhanced efficiency of gene targeting relative to the parental strain. Finally, we show the implementation of Gateway technology and a single-step PCR strategy to create deletions in H. polymorpha.

AB - Hansenula polymorpha is an important yeast in industrial biotechnology. In addition, it is extensively used in fundamental research devoted to unravel the principles of peroxisome biology and nitrate assimilation. Here we present an overview of key components of the genetic toolbox for H. polymorpha. In addition, we present new selection markers that we recently implemented in H. polymorpha. We describe novel strategies for the efficient creation of targeted gene deletions and integrations in H. polymorpha. For this, we generated a similar to 80 mutant, deficient in non-homologous end joining, resulting in strongly enhanced efficiency of gene targeting relative to the parental strain. Finally, we show the implementation of Gateway technology and a single-step PCR strategy to create deletions in H. polymorpha.

KW - dominant markers

KW - Gateway technology

KW - genetic tools

KW - methylotrophic yeast

KW - non-homologous end-joining

KW - YEAST EXPRESSION PLATFORMS

KW - MATRIX PROTEIN IMPORT

KW - SACCHAROMYCES-CEREVISIAE

KW - PEROXISOMAL MEMBRANE

KW - METHYLOTROPHIC YEAST

KW - ARXULA-ADENINIVORANS

KW - PICHIA-PASTORIS

KW - ALCOHOL OXIDASE

KW - METHANOL

KW - FISSION

U2 - 10.1111/j.1567-1364.2011.00772.x

DO - 10.1111/j.1567-1364.2011.00772.x

M3 - Review article

C2 - 22129301

VL - 12

SP - 271

EP - 278

JO - Fems Yeast Research

JF - Fems Yeast Research

SN - 1567-1356

IS - 3

ER -

ID: 5541446

University of Groningen staff: edit these data