Publication

Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers

Op den Dries, S., Karimian, N., Westerkamp, A. C., Sutton, M. E., Kuipers, M., Wiersema-Buist, J., Ottens, P. J., Kuipers, J., Giepmans, B. N., Leuvenink, H. G. D., Lisman, T. & Porte, R. J., Jul-2016, In : Liver Transplantation. 22, 7, p. 994-1005 12 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Op den Dries, S., Karimian, N., Westerkamp, A. C., Sutton, M. E., Kuipers, M., Wiersema-Buist, J., ... Porte, R. J. (2016). Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers. Liver Transplantation, 22(7), 994-1005. https://doi.org/10.1002/lt.24436

Author

Op den Dries, Sanna ; Karimian, Negin ; Westerkamp, Andrie C ; Sutton, Michael E ; Kuipers, Michiel ; Wiersema-Buist, Janneke ; Ottens, Petra J ; Kuipers, Jeroen ; Giepmans, Ben N ; Leuvenink, Henri G D ; Lisman, Ton ; Porte, Robert J. / Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers. In: Liver Transplantation. 2016 ; Vol. 22, No. 7. pp. 994-1005.

Harvard

Op den Dries, S, Karimian, N, Westerkamp, AC, Sutton, ME, Kuipers, M, Wiersema-Buist, J, Ottens, PJ, Kuipers, J, Giepmans, BN, Leuvenink, HGD, Lisman, T & Porte, RJ 2016, 'Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers', Liver Transplantation, vol. 22, no. 7, pp. 994-1005. https://doi.org/10.1002/lt.24436

Standard

Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers. / Op den Dries, Sanna; Karimian, Negin; Westerkamp, Andrie C; Sutton, Michael E; Kuipers, Michiel; Wiersema-Buist, Janneke; Ottens, Petra J; Kuipers, Jeroen; Giepmans, Ben N; Leuvenink, Henri G D; Lisman, Ton; Porte, Robert J.

In: Liver Transplantation, Vol. 22, No. 7, 07.2016, p. 994-1005.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Op den Dries S, Karimian N, Westerkamp AC, Sutton ME, Kuipers M, Wiersema-Buist J et al. Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers. Liver Transplantation. 2016 Jul;22(7):994-1005. https://doi.org/10.1002/lt.24436


BibTeX

@article{f2d15826be224ea58e0567bc37da87cc,
title = "Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers",
abstract = "BACKGROUND: Bile duct injury may occur during liver procurement and transplantation, especially in livers donated after circulatory death (DCD). Normothermic machine perfusion (NMP) has been shown to reduce hepatic injury, compared to static cold storage (SCS). However, it is unknown whether NMP provides better preservation of bile ducts.AIM: The aim of this study was to determine the impact of NMP on bile duct preservation in both DCD and non-DCD livers.METHODS: DCD and non-DCD livers obtained from Lewis rats were preserved for 3h using either SCS or NMP, followed by 2h ex-vivo reperfusion.RESULTS: Biomarkers of bile duct injury (γ-GT and LDH in bile) were lower in NMP preserved livers, compared to SCS preserved livers. Biliary bicarbonate concentration, reflecting biliary epithelial function, was 2-fold higher in NMP preserved livers (p<0.01). In parallel with this, pH of bile was significantly higher in NMP preserved livers (7.63±0.02 and 7.74±0.05, for non-DCD and DCD livers, respectively), compared with SCS (7.46±0.02 and 7.49±0.04, for non-DCD and DCD livers, respectively). Scanning and transmission electron microscopy of donor extrahepatic bile ducts demonstrated significantly decreased injury of the biliary epithelium of NMP preserved donor livers (including the loss of lateral interdigitations and mitrochondrial injury). Differences between NMP and SCS were most prominent in DCD livers.CONCLUSION: Compared to conventional SCS, NMP provides superior preservation of bile duct epithelial cell function and morphology, especially in DCD donor livers. By reducing biliary injury, NMP could have an important impact on the utilization of DCD livers and outcome after transplantation. This article is protected by copyright. All rights reserved.",
keywords = "CARDIAC DEATH, SALT TOXICITY, HCO3-UMBRELLA, COLD-STORAGE, RISK-FACTORS, TRANSPLANTATION, PRESERVATION, STRICTURES, ISCHEMIA, CHOLANGIOCYTES",
author = "{Op den Dries}, Sanna and Negin Karimian and Westerkamp, {Andrie C} and Sutton, {Michael E} and Michiel Kuipers and Janneke Wiersema-Buist and Ottens, {Petra J} and Jeroen Kuipers and Giepmans, {Ben N} and Leuvenink, {Henri G D} and Ton Lisman and Porte, {Robert J}",
note = "{\circledC} 2016 American Association for the Study of Liver Diseases.",
year = "2016",
month = "7",
doi = "10.1002/lt.24436",
language = "English",
volume = "22",
pages = "994--1005",
journal = "Liver Transplantation",
issn = "1527-6465",
publisher = "Wiley",
number = "7",

}

RIS

TY - JOUR

T1 - Normothermic Machine Perfusion Reduces Bile Duct Injury and Improves Biliary Epithelial Function in Rat Donor Livers

AU - Op den Dries, Sanna

AU - Karimian, Negin

AU - Westerkamp, Andrie C

AU - Sutton, Michael E

AU - Kuipers, Michiel

AU - Wiersema-Buist, Janneke

AU - Ottens, Petra J

AU - Kuipers, Jeroen

AU - Giepmans, Ben N

AU - Leuvenink, Henri G D

AU - Lisman, Ton

AU - Porte, Robert J

N1 - © 2016 American Association for the Study of Liver Diseases.

PY - 2016/7

Y1 - 2016/7

N2 - BACKGROUND: Bile duct injury may occur during liver procurement and transplantation, especially in livers donated after circulatory death (DCD). Normothermic machine perfusion (NMP) has been shown to reduce hepatic injury, compared to static cold storage (SCS). However, it is unknown whether NMP provides better preservation of bile ducts.AIM: The aim of this study was to determine the impact of NMP on bile duct preservation in both DCD and non-DCD livers.METHODS: DCD and non-DCD livers obtained from Lewis rats were preserved for 3h using either SCS or NMP, followed by 2h ex-vivo reperfusion.RESULTS: Biomarkers of bile duct injury (γ-GT and LDH in bile) were lower in NMP preserved livers, compared to SCS preserved livers. Biliary bicarbonate concentration, reflecting biliary epithelial function, was 2-fold higher in NMP preserved livers (p<0.01). In parallel with this, pH of bile was significantly higher in NMP preserved livers (7.63±0.02 and 7.74±0.05, for non-DCD and DCD livers, respectively), compared with SCS (7.46±0.02 and 7.49±0.04, for non-DCD and DCD livers, respectively). Scanning and transmission electron microscopy of donor extrahepatic bile ducts demonstrated significantly decreased injury of the biliary epithelium of NMP preserved donor livers (including the loss of lateral interdigitations and mitrochondrial injury). Differences between NMP and SCS were most prominent in DCD livers.CONCLUSION: Compared to conventional SCS, NMP provides superior preservation of bile duct epithelial cell function and morphology, especially in DCD donor livers. By reducing biliary injury, NMP could have an important impact on the utilization of DCD livers and outcome after transplantation. This article is protected by copyright. All rights reserved.

AB - BACKGROUND: Bile duct injury may occur during liver procurement and transplantation, especially in livers donated after circulatory death (DCD). Normothermic machine perfusion (NMP) has been shown to reduce hepatic injury, compared to static cold storage (SCS). However, it is unknown whether NMP provides better preservation of bile ducts.AIM: The aim of this study was to determine the impact of NMP on bile duct preservation in both DCD and non-DCD livers.METHODS: DCD and non-DCD livers obtained from Lewis rats were preserved for 3h using either SCS or NMP, followed by 2h ex-vivo reperfusion.RESULTS: Biomarkers of bile duct injury (γ-GT and LDH in bile) were lower in NMP preserved livers, compared to SCS preserved livers. Biliary bicarbonate concentration, reflecting biliary epithelial function, was 2-fold higher in NMP preserved livers (p<0.01). In parallel with this, pH of bile was significantly higher in NMP preserved livers (7.63±0.02 and 7.74±0.05, for non-DCD and DCD livers, respectively), compared with SCS (7.46±0.02 and 7.49±0.04, for non-DCD and DCD livers, respectively). Scanning and transmission electron microscopy of donor extrahepatic bile ducts demonstrated significantly decreased injury of the biliary epithelium of NMP preserved donor livers (including the loss of lateral interdigitations and mitrochondrial injury). Differences between NMP and SCS were most prominent in DCD livers.CONCLUSION: Compared to conventional SCS, NMP provides superior preservation of bile duct epithelial cell function and morphology, especially in DCD donor livers. By reducing biliary injury, NMP could have an important impact on the utilization of DCD livers and outcome after transplantation. This article is protected by copyright. All rights reserved.

KW - CARDIAC DEATH

KW - SALT TOXICITY

KW - HCO3-UMBRELLA

KW - COLD-STORAGE

KW - RISK-FACTORS

KW - TRANSPLANTATION

KW - PRESERVATION

KW - STRICTURES

KW - ISCHEMIA

KW - CHOLANGIOCYTES

U2 - 10.1002/lt.24436

DO - 10.1002/lt.24436

M3 - Article

C2 - 26946466

VL - 22

SP - 994

EP - 1005

JO - Liver Transplantation

JF - Liver Transplantation

SN - 1527-6465

IS - 7

ER -

ID: 31383489