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Normal peroxisome development from vesicles induced by truncated Hansenula polymorpha Pex3p

Faber, KN., Haan, GJ., Baerends, RJS., Kram, AM. & Veenhuis, M., 29-Mar-2002, In : The Journal of Biological Chemistry. 277, 13, p. 11026-11033 8 p.

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DOI

We show that the synthesis of the N-terminal 50 amino acids of Pex3p (Pex3p(1-50)) in Hansenula polymorpha pex3 cells is associated with the formation of vesicular membrane structures. Biochemical and ultrastructural findings suggest that the nuclear membrane is the donor membrane compartment of these vesicles. These structures also contain Pex14p and can develop into functional peroxisomes after subsequent reintroduction of the full-length Pex3p protein. We discuss the significance of this finding in relation to peroxisome reintroduction, e.g. in case peroxisomes are lost due to failure in inheritance.

Original languageEnglish
Pages (from-to)11026-11033
Number of pages8
JournalThe Journal of Biological Chemistry
Volume277
Issue number13
Publication statusPublished - 29-Mar-2002

    Keywords

  • DIHYDROXYACETONE SYNTHASE, SELECTIVE INACTIVATION, ENDOPLASMIC-RETICULUM, DEFICIENT MUTANTS, TARGETING SIGNAL, PICHIA-PASTORIS, ALCOHOL OXIDASE, N-TERMINUS, BIOGENESIS, MEMBRANE

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