Noninvasive optical and nuclear imaging of Staphylococcus-specific infection with a human monoclonal antibody-based probePastrana, F. R., Thompson, J. M., Heuker, M., Hoekstra, H., Dillen, C. A., Ortines, R. V., Ashbaugh, A. G., Pickett, J. E., Linssen, M. D., Bernthal, N. M., Francis, K. P., Buist, G., van Oosten, M., van Dam, G. M., Thorek, D. L. J., Miller, L. S. & van Dijl, J. M., Jan-2018, In : Virulence. 9, 1, p. 262-272 11 p.
Research output: Contribution to journal › Article › Academic › peer-review
Staphylococcus aureus infections are a major threat in healthcare, requiring adequate early-stage diagnosis and treatment. This calls for novel diagnostic tools that allow noninvasive in vivo detection of staphylococci. Here we performed a preclinical study to investigate a novel fully-human monoclonal antibody 1D9 that specifically targets the immunodominant staphylococcal antigen A (IsaA). We show that 1D9 binds invariantly to S. aureus cells and may further target other staphylococcal species. Importantly, using a human post-mortem implant model and an in vivo murine skin infection model, preclinical feasibility was demonstrated for 1D9 labeled with the near-infrared fluorophore IRDye800CW to be applied for direct optical imaging of in vivo S. aureus infections. Additionally, (89)Zirconium-labeled 1D9 could be used for positron emission tomography imaging of an in vivo S. aureus thigh infection model. Our findings pave the way towards clinical implementation of targeted imaging of staphylococcal infections using the human monoclonal antibody
|Number of pages||11|
|Publication status||Published - Jan-2018|
- human monoclonal antibody, immunodominant staphylococcal antigen A, IsaA, PET, Zr-89, Staphylococcus aureus, BACTERIAL-INFECTIONS, IN-VIVO, AUREUS INFECTIONS, TUBERCULOSIS, ACTIVATION, MECHANISMS, RESISTANCE, DETECT, MICE, ISAA