NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell LymphomaKluiver, J., Niu, F., Yuan, Y., Kok, K., van den Berg, A. & Dzikiewicz-Krawczyk, A., 19-Feb-2019, Lymphoma. Küppers, R. (ed.). Springer, Vol. 1956. p. 269-282 14 p. (Methods in Molecular Biology).
Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
MicroRNAs (miRNAs) play important roles in development, differentiation, and homeostasis by regulating protein translation. In B-cell lymphoma, many miRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in the control of cell growth. First, we provide a protocol for performing high-throughput screening for miRNA inhibition and overexpression. Second, we describe the procedure for next-generation sequencing library preparation. Third, we provide a workflow for data analysis.
|Title of host publication||Lymphoma|
|Number of pages||14|
|Publication status||E-pub ahead of print - 19-Feb-2019|
|Name||Methods in Molecular Biology|