Neodymium as an alternative contrast for uranium in electron microscopy

Kuipers, J. & Giepmans, B. N. G., Apr-2020, In : Histochemistry and cell biology. 153, 4, p. 271-277 7 p.

Research output: Contribution to journalArticleAcademicpeer-review

Uranyl acetate is the standard contrasting agent in electron microscopy (EM), but it is toxic and radioactive. We reasoned neodymium acetate might substitute uranyl acetate as a contrasting agent, and we find that neodymium acetate indeed can replace uranyl acetate in several routine applications. Since neodymium acetate is not toxic, not radioactive and easy to use, we foresee neodymium will replace uranyl in many EM sample preparation applications worldwide.

Original languageEnglish
Pages (from-to)271-277
Number of pages7
JournalHistochemistry and cell biology
Issue number4
Publication statusPublished - Apr-2020


  • Contrast, Electron microscopy, Neodymium, Alternative contrast, Neodymium acetate, Uranyl acetate, OOLONG TEA EXTRACT, URANYL ACETATE, ULTRATHIN SECTIONS, TISSUE SECTIONS, SUBSTITUTE, LEAD

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