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Nasal gene expression differentiates COPD from controls and overlaps bronchial gene expression

Boudewijn, I. M., Faiz, A., Steiling, K., van der Wiel, E., Telenga, E. D., Hoonhorst, S. J. M., ten Hacken, N. H. T., Brandsma, C-A., Kerstjens, H. A. M., Timens, W., Heijink, I. H., Jonker, M. R., de Bruin, H. G., Vroegop, J. S., Pasma, H. R., Boersma, W. G., Wielders, P., van den Elshout, F., Mansour, K., Spira, A., Lenburg, M. E., Guryev, V., Postma, D. S. & van den Berge, M., 21-Dec-2017, In : Respiratory Research. 18, 10 p., 213.

Research output: Contribution to journalArticleAcademicpeer-review

Background: Nasal gene expression profiling is a promising method to characterize COPD non-invasively. We aimed to identify a nasal gene expression profile to distinguish COPD patients from healthy controls. We investigated whether this COPD-associated gene expression profile in nasal epithelium is comparable with the profile observed in bronchial epithelium.

Methods: Genome wide gene expression analysis was performed on nasal epithelial brushes of 31 severe COPD patients and 22 controls, all current smokers, using Affymetrix Human Gene 1.0 ST Arrays. We repeated the gene expression analysis on bronchial epithelial brushes in 2 independent cohorts of mild-to-moderate COPD patients and controls.

Results: In nasal epithelium, 135 genes were significantly differentially expressed between severe COPD patients and controls, 21 being up-and 114 downregulated in COPD (false discovery rate <0.01). Gene Set Enrichment Analysis (GSEA) showed significant concordant enrichment of COPD-associated nasal and bronchial gene expression in both independent cohorts (FDRGSEA <0.001).

Conclusion: We identified a nasal gene expression profile that differentiates severe COPD patients from controls. Of interest, part of the nasal gene expression changes in COPD mimics differentially expressed genes in the bronchus. These findings indicate that nasal gene expression profiling is potentially useful as a non-invasive biomarker in COPD.

Original languageEnglish
Article number213
Number of pages10
JournalRespiratory Research
Volume18
Publication statusPublished - 21-Dec-2017

    Keywords

  • COPD, Nasal epithelium, Bronchial epithelium, Genome wide gene expression, Microarray, OBSTRUCTIVE PULMONARY-DISEASE, ENRICHMENT ANALYSIS, LUNG FIBROBLASTS, EMPHYSEMA, STANDARDIZATION, BIOSYNTHESIS, PATHWAYS, SMOKING, PROTEIN, CILIA

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