Publication

Nanodiamonds as multi-purpose labels for microscopy

Hemelaar, S. R., de Boer, P., Chipaux, M., Zuidema, W., Hamoh, T., Martinez, F. P., Nagl, A., Hoogenboom, J. P., Giepmans, B. N. G. & Schirhagl, R., 7-Apr-2017, In : Scientific Reports. 7, 1, 9 p., 720.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Hemelaar, S. R., de Boer, P., Chipaux, M., Zuidema, W., Hamoh, T., Martinez, F. P., ... Schirhagl, R. (2017). Nanodiamonds as multi-purpose labels for microscopy. Scientific Reports, 7(1), [720]. https://doi.org/10.1038/s41598-017-00797-2

Author

Hemelaar, S. R. ; de Boer, P. ; Chipaux, M. ; Zuidema, W. ; Hamoh, T. ; Martinez, F. Perona ; Nagl, A. ; Hoogenboom, J. P. ; Giepmans, B. N. G. ; Schirhagl, R. / Nanodiamonds as multi-purpose labels for microscopy. In: Scientific Reports. 2017 ; Vol. 7, No. 1.

Harvard

Hemelaar, SR, de Boer, P, Chipaux, M, Zuidema, W, Hamoh, T, Martinez, FP, Nagl, A, Hoogenboom, JP, Giepmans, BNG & Schirhagl, R 2017, 'Nanodiamonds as multi-purpose labels for microscopy', Scientific Reports, vol. 7, no. 1, 720. https://doi.org/10.1038/s41598-017-00797-2

Standard

Nanodiamonds as multi-purpose labels for microscopy. / Hemelaar, S. R.; de Boer, P.; Chipaux, M.; Zuidema, W.; Hamoh, T.; Martinez, F. Perona; Nagl, A.; Hoogenboom, J. P.; Giepmans, B. N. G.; Schirhagl, R.

In: Scientific Reports, Vol. 7, No. 1, 720, 07.04.2017.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Hemelaar SR, de Boer P, Chipaux M, Zuidema W, Hamoh T, Martinez FP et al. Nanodiamonds as multi-purpose labels for microscopy. Scientific Reports. 2017 Apr 7;7(1). 720. https://doi.org/10.1038/s41598-017-00797-2


BibTeX

@article{033e6daf6afe43939dd4113be74de174,
title = "Nanodiamonds as multi-purpose labels for microscopy",
abstract = "Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite fluorescence using a focused electron beam (cathodoluminescence; CL) for high-resolution localization; and (iii) the potential use for nanoscale sensing. For all these schemes, the development of versatile molecular labeling using relatively small diamonds is essential. Here, we show the direct targeting of a biological molecule with nanodiamonds as small as 70 nm using a streptavidin conjugation and standard antibody labelling approach. We also show internalization of 40 nm sized nanodiamonds. The fluorescence from the nanodiamonds survives osmium-fixation and plastic embedding making them suited for correlative light and electron microscopy. We show that CL can be observed from epon-embedded nanodiamonds, while surface-exposed nanoparticles also stand out in secondary electron (SE) signal due to the exceptionally high diamond SE yield. Finally, we demonstrate the magnetic read-out using fluorescence from diamonds prior to embedding. Thus, our results firmly establish nanodiamonds containing nitrogen-vacancy centers as unique, versatile probes for combining and correlating different types of microscopy, from fluorescence imaging and magnetometry to ultrastructural investigation using electron microscopy.",
keywords = "ELECTRON-MICROSCOPY, FLUORESCENT NANODIAMONDS, CATHODOLUMINESCENCE MICROSCOPY, CORRELATED FLUORESCENCE, QUANTUM DOTS, DIAMOND, LIGHT, CELLS, NANOPHOSPHORS, CENTERS",
author = "Hemelaar, {S. R.} and {de Boer}, P. and M. Chipaux and W. Zuidema and T. Hamoh and Martinez, {F. Perona} and A. Nagl and Hoogenboom, {J. P.} and Giepmans, {B. N. G.} and R. Schirhagl",
year = "2017",
month = "4",
day = "7",
doi = "10.1038/s41598-017-00797-2",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Nanodiamonds as multi-purpose labels for microscopy

AU - Hemelaar, S. R.

AU - de Boer, P.

AU - Chipaux, M.

AU - Zuidema, W.

AU - Hamoh, T.

AU - Martinez, F. Perona

AU - Nagl, A.

AU - Hoogenboom, J. P.

AU - Giepmans, B. N. G.

AU - Schirhagl, R.

PY - 2017/4/7

Y1 - 2017/4/7

N2 - Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite fluorescence using a focused electron beam (cathodoluminescence; CL) for high-resolution localization; and (iii) the potential use for nanoscale sensing. For all these schemes, the development of versatile molecular labeling using relatively small diamonds is essential. Here, we show the direct targeting of a biological molecule with nanodiamonds as small as 70 nm using a streptavidin conjugation and standard antibody labelling approach. We also show internalization of 40 nm sized nanodiamonds. The fluorescence from the nanodiamonds survives osmium-fixation and plastic embedding making them suited for correlative light and electron microscopy. We show that CL can be observed from epon-embedded nanodiamonds, while surface-exposed nanoparticles also stand out in secondary electron (SE) signal due to the exceptionally high diamond SE yield. Finally, we demonstrate the magnetic read-out using fluorescence from diamonds prior to embedding. Thus, our results firmly establish nanodiamonds containing nitrogen-vacancy centers as unique, versatile probes for combining and correlating different types of microscopy, from fluorescence imaging and magnetometry to ultrastructural investigation using electron microscopy.

AB - Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite fluorescence using a focused electron beam (cathodoluminescence; CL) for high-resolution localization; and (iii) the potential use for nanoscale sensing. For all these schemes, the development of versatile molecular labeling using relatively small diamonds is essential. Here, we show the direct targeting of a biological molecule with nanodiamonds as small as 70 nm using a streptavidin conjugation and standard antibody labelling approach. We also show internalization of 40 nm sized nanodiamonds. The fluorescence from the nanodiamonds survives osmium-fixation and plastic embedding making them suited for correlative light and electron microscopy. We show that CL can be observed from epon-embedded nanodiamonds, while surface-exposed nanoparticles also stand out in secondary electron (SE) signal due to the exceptionally high diamond SE yield. Finally, we demonstrate the magnetic read-out using fluorescence from diamonds prior to embedding. Thus, our results firmly establish nanodiamonds containing nitrogen-vacancy centers as unique, versatile probes for combining and correlating different types of microscopy, from fluorescence imaging and magnetometry to ultrastructural investigation using electron microscopy.

KW - ELECTRON-MICROSCOPY

KW - FLUORESCENT NANODIAMONDS

KW - CATHODOLUMINESCENCE MICROSCOPY

KW - CORRELATED FLUORESCENCE

KW - QUANTUM DOTS

KW - DIAMOND

KW - LIGHT

KW - CELLS

KW - NANOPHOSPHORS

KW - CENTERS

U2 - 10.1038/s41598-017-00797-2

DO - 10.1038/s41598-017-00797-2

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 720

ER -

ID: 41260808