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Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment

Sigaeva, A., Morita, A., Hemelaar, S. R. & Schirhagl, R., 7-Oct-2019, In : Nanoscale. 11, 37, p. 17357-17367 11 p.

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  • Nanodiamond uptake in colon cancer cells the influence of direction and trypsin-EDTA treatment

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DOI

Nanoparticles are routinely used in cell biology. They deliver drugs or function as labels or sensors. For many of these applications it is essential that the nanoparticles enter the cells. While some cell types readily ingest all kinds of particles, others just don't. We report that uptake can be enhanced for some cells if the particles are administered from the basolateral side of the cells (in this case from below). Compared to apical uptake (from above), we report an 8-fold increase in the number of fluorescent nanodiamonds internalized by the colon cancer cell line HT29. Up to 96% of the cells treated by a modified protocol contain at least one nanodiamond, whereas in the control group we could observe nanodiamonds in less than half of the cells. We were also able to show that simple treatment of cell clusters with trypsin-EDTA leads to the same enhancement of the nanodiamond uptake as seeding the cells on top of the nanoparticles. Although our study is focused on nanodiamonds in HT29 cells, we believe that this method could also be applicable for other nanoparticles and cells with a specific directionality.

Original languageEnglish
Pages (from-to)17357-17367
Number of pages11
JournalNanoscale
Volume11
Issue number37
Publication statusPublished - 7-Oct-2019

    Keywords

  • CONJUGATED FLUORESCENT NANODIAMONDS, CELLULAR UPTAKE, FUNCTIONALIZATION, TOPOGRAPHY

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