Publication

Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment

Sigaeva, A., Morita, A., Hemelaar, S. R. & Schirhagl, R., 7-Oct-2019, In : Nanoscale. 11, 37, p. 17357-17367 11 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Sigaeva, A., Morita, A., Hemelaar, S. R., & Schirhagl, R. (2019). Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment. Nanoscale, 11(37), 17357-17367. https://doi.org/10.1039/c9nr04228h

Author

Sigaeva, Alina ; Morita, Aryan ; Hemelaar, Simon R. ; Schirhagl, R. / Nanodiamond uptake in colon cancer cells : the influence of direction and trypsin-EDTA treatment. In: Nanoscale. 2019 ; Vol. 11, No. 37. pp. 17357-17367.

Harvard

Sigaeva, A, Morita, A, Hemelaar, SR & Schirhagl, R 2019, 'Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment', Nanoscale, vol. 11, no. 37, pp. 17357-17367. https://doi.org/10.1039/c9nr04228h

Standard

Nanodiamond uptake in colon cancer cells : the influence of direction and trypsin-EDTA treatment. / Sigaeva, Alina; Morita, Aryan; Hemelaar, Simon R.; Schirhagl, R.

In: Nanoscale, Vol. 11, No. 37, 07.10.2019, p. 17357-17367.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Sigaeva A, Morita A, Hemelaar SR, Schirhagl R. Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment. Nanoscale. 2019 Oct 7;11(37):17357-17367. https://doi.org/10.1039/c9nr04228h


BibTeX

@article{ed54d4c0c1e54eccb5ee7805ef272b93,
title = "Nanodiamond uptake in colon cancer cells: the influence of direction and trypsin-EDTA treatment",
abstract = "Nanoparticles are routinely used in cell biology. They deliver drugs or function as labels or sensors. For many of these applications it is essential that the nanoparticles enter the cells. While some cell types readily ingest all kinds of particles, others just don't. We report that uptake can be enhanced for some cells if the particles are administered from the basolateral side of the cells (in this case from below). Compared to apical uptake (from above), we report an 8-fold increase in the number of fluorescent nanodiamonds internalized by the colon cancer cell line HT29. Up to 96{\%} of the cells treated by a modified protocol contain at least one nanodiamond, whereas in the control group we could observe nanodiamonds in less than half of the cells. We were also able to show that simple treatment of cell clusters with trypsin-EDTA leads to the same enhancement of the nanodiamond uptake as seeding the cells on top of the nanoparticles. Although our study is focused on nanodiamonds in HT29 cells, we believe that this method could also be applicable for other nanoparticles and cells with a specific directionality.",
keywords = "CONJUGATED FLUORESCENT NANODIAMONDS, CELLULAR UPTAKE, FUNCTIONALIZATION, TOPOGRAPHY",
author = "Alina Sigaeva and Aryan Morita and Hemelaar, {Simon R.} and R. Schirhagl",
year = "2019",
month = "10",
day = "7",
doi = "10.1039/c9nr04228h",
language = "English",
volume = "11",
pages = "17357--17367",
journal = "Nanoscale",
issn = "2040-3364",
publisher = "ROYAL SOC CHEMISTRY",
number = "37",

}

RIS

TY - JOUR

T1 - Nanodiamond uptake in colon cancer cells

T2 - the influence of direction and trypsin-EDTA treatment

AU - Sigaeva, Alina

AU - Morita, Aryan

AU - Hemelaar, Simon R.

AU - Schirhagl, R.

PY - 2019/10/7

Y1 - 2019/10/7

N2 - Nanoparticles are routinely used in cell biology. They deliver drugs or function as labels or sensors. For many of these applications it is essential that the nanoparticles enter the cells. While some cell types readily ingest all kinds of particles, others just don't. We report that uptake can be enhanced for some cells if the particles are administered from the basolateral side of the cells (in this case from below). Compared to apical uptake (from above), we report an 8-fold increase in the number of fluorescent nanodiamonds internalized by the colon cancer cell line HT29. Up to 96% of the cells treated by a modified protocol contain at least one nanodiamond, whereas in the control group we could observe nanodiamonds in less than half of the cells. We were also able to show that simple treatment of cell clusters with trypsin-EDTA leads to the same enhancement of the nanodiamond uptake as seeding the cells on top of the nanoparticles. Although our study is focused on nanodiamonds in HT29 cells, we believe that this method could also be applicable for other nanoparticles and cells with a specific directionality.

AB - Nanoparticles are routinely used in cell biology. They deliver drugs or function as labels or sensors. For many of these applications it is essential that the nanoparticles enter the cells. While some cell types readily ingest all kinds of particles, others just don't. We report that uptake can be enhanced for some cells if the particles are administered from the basolateral side of the cells (in this case from below). Compared to apical uptake (from above), we report an 8-fold increase in the number of fluorescent nanodiamonds internalized by the colon cancer cell line HT29. Up to 96% of the cells treated by a modified protocol contain at least one nanodiamond, whereas in the control group we could observe nanodiamonds in less than half of the cells. We were also able to show that simple treatment of cell clusters with trypsin-EDTA leads to the same enhancement of the nanodiamond uptake as seeding the cells on top of the nanoparticles. Although our study is focused on nanodiamonds in HT29 cells, we believe that this method could also be applicable for other nanoparticles and cells with a specific directionality.

KW - CONJUGATED FLUORESCENT NANODIAMONDS

KW - CELLULAR UPTAKE

KW - FUNCTIONALIZATION

KW - TOPOGRAPHY

U2 - 10.1039/c9nr04228h

DO - 10.1039/c9nr04228h

M3 - Article

VL - 11

SP - 17357

EP - 17367

JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 37

ER -

ID: 95172799