Publication

Nanodiamond for Sample Preparation in Proteomics

Perona Martinez, F., Nagl, A., Guluzade, S. & Schirhagl, R., 6-Aug-2019, In : Analytical Chemistry. 91, 15, p. 9800-9805 6 p.

Research output: Contribution to journalArticleAcademicpeer-review

APA

Perona Martinez, F., Nagl, A., Guluzade, S., & Schirhagl, R. (2019). Nanodiamond for Sample Preparation in Proteomics. Analytical Chemistry, 91(15), 9800-9805. https://doi.org/10.1021/acs.analchem.9b01459

Author

Perona Martinez, Felipe ; Nagl, Andreas ; Guluzade, Sona ; Schirhagl, Romana. / Nanodiamond for Sample Preparation in Proteomics. In: Analytical Chemistry. 2019 ; Vol. 91, No. 15. pp. 9800-9805.

Harvard

Perona Martinez, F, Nagl, A, Guluzade, S & Schirhagl, R 2019, 'Nanodiamond for Sample Preparation in Proteomics', Analytical Chemistry, vol. 91, no. 15, pp. 9800-9805. https://doi.org/10.1021/acs.analchem.9b01459

Standard

Nanodiamond for Sample Preparation in Proteomics. / Perona Martinez, Felipe; Nagl, Andreas; Guluzade, Sona; Schirhagl, Romana.

In: Analytical Chemistry, Vol. 91, No. 15, 06.08.2019, p. 9800-9805.

Research output: Contribution to journalArticleAcademicpeer-review

Vancouver

Perona Martinez F, Nagl A, Guluzade S, Schirhagl R. Nanodiamond for Sample Preparation in Proteomics. Analytical Chemistry. 2019 Aug 6;91(15):9800-9805. https://doi.org/10.1021/acs.analchem.9b01459


BibTeX

@article{94afb2fdae8445448dc4a2254a6beb53,
title = "Nanodiamond for Sample Preparation in Proteomics",
abstract = "Protein analysis of potential disease markers in blood is complicated by the fact that proteins in plasma show very different abundances. As a result, high-abundance proteins dominate the analysis, which often render the analysis of low-abundance proteins impossible. Depleting high-abundance proteins is one strategy to solve this problem. Here, we present, for the first time, a very simple approach based on selective binding of serum proteins to the surface of nanodiamonds. In our first proof-of-principle experiments, we were able to detect, on average, eight proteins that are present at a concentration of 1 ng/mL (instead of 0.5 ng/mL in the control without sample preparation). Remarkably, we detect proteins down to a concentration of 400 pg/mL after only one simple depletion step. Among the proteins we could analyze are also numerous disease biomarkers, including markers for multiple cancer forms, cardiovascular diseases, or Alzheimer's disease. Remarkably, many of the biomarkers we find also could not be detected with a state-of-the-art ultrahigh-performance liquid chromatography column (which depletes the 64 most-abundant serum proteins).",
author = "{Perona Martinez}, Felipe and Andreas Nagl and Sona Guluzade and Romana Schirhagl",
year = "2019",
month = "8",
day = "6",
doi = "10.1021/acs.analchem.9b01459",
language = "English",
volume = "91",
pages = "9800--9805",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "AMER CHEMICAL SOC INC",
number = "15",

}

RIS

TY - JOUR

T1 - Nanodiamond for Sample Preparation in Proteomics

AU - Perona Martinez, Felipe

AU - Nagl, Andreas

AU - Guluzade, Sona

AU - Schirhagl, Romana

PY - 2019/8/6

Y1 - 2019/8/6

N2 - Protein analysis of potential disease markers in blood is complicated by the fact that proteins in plasma show very different abundances. As a result, high-abundance proteins dominate the analysis, which often render the analysis of low-abundance proteins impossible. Depleting high-abundance proteins is one strategy to solve this problem. Here, we present, for the first time, a very simple approach based on selective binding of serum proteins to the surface of nanodiamonds. In our first proof-of-principle experiments, we were able to detect, on average, eight proteins that are present at a concentration of 1 ng/mL (instead of 0.5 ng/mL in the control without sample preparation). Remarkably, we detect proteins down to a concentration of 400 pg/mL after only one simple depletion step. Among the proteins we could analyze are also numerous disease biomarkers, including markers for multiple cancer forms, cardiovascular diseases, or Alzheimer's disease. Remarkably, many of the biomarkers we find also could not be detected with a state-of-the-art ultrahigh-performance liquid chromatography column (which depletes the 64 most-abundant serum proteins).

AB - Protein analysis of potential disease markers in blood is complicated by the fact that proteins in plasma show very different abundances. As a result, high-abundance proteins dominate the analysis, which often render the analysis of low-abundance proteins impossible. Depleting high-abundance proteins is one strategy to solve this problem. Here, we present, for the first time, a very simple approach based on selective binding of serum proteins to the surface of nanodiamonds. In our first proof-of-principle experiments, we were able to detect, on average, eight proteins that are present at a concentration of 1 ng/mL (instead of 0.5 ng/mL in the control without sample preparation). Remarkably, we detect proteins down to a concentration of 400 pg/mL after only one simple depletion step. Among the proteins we could analyze are also numerous disease biomarkers, including markers for multiple cancer forms, cardiovascular diseases, or Alzheimer's disease. Remarkably, many of the biomarkers we find also could not be detected with a state-of-the-art ultrahigh-performance liquid chromatography column (which depletes the 64 most-abundant serum proteins).

U2 - 10.1021/acs.analchem.9b01459

DO - 10.1021/acs.analchem.9b01459

M3 - Article

VL - 91

SP - 9800

EP - 9805

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 15

ER -

ID: 92915720